A luminescence switch-on assay for the detection of specific gene deletion using G-quadruplex DNA and silver nanoclusters

Modi Wang; Wanhe Wang; Chenfu LIU; Jinbiao Liu; Tian Shu Kang; Chung Hang Leung; Edmond Dik Lung MA

doi:10.1039/c6qm00068a

A luminescence switch-on assay for the detection of specific gene deletion using G-quadruplex DNA and silver nanoclusters

Modi Wang
, Wanhe Wang
, Chenfu LIU
, Jinbiao Liu
, Tian Shu Kang
, Chung Hang Leung
, Edmond Dik Lung MA^*

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

16 Citations (Scopus)

Abstract

A switch-on gene deletion detection platform is reported based on the alteration of the effective distance between G-quadruplex DNA and silver nanoclusters (AgNCs). Moreover, the role of the G-quadruplex structure in interacting with AgNCs has also been demonstrated. The detection of the deletion mutants of LMP1 and CCR5 genes is performed as the proof-of-concept of our assay. Finally, the potential biological application of the assay was demonstrated in 10% v/v human serum.

Original language	English
Pages (from-to)	128-131
Number of pages	4
Journal	Materials Chemistry Frontiers
Volume	1
Issue number	1
DOIs	https://doi.org/10.1039/c6qm00068a
Publication status	Published - Jan 2017

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@article{1698236a03ae4af1bcf96104ebcd9239,

title = "A luminescence switch-on assay for the detection of specific gene deletion using G-quadruplex DNA and silver nanoclusters",

abstract = "A switch-on gene deletion detection platform is reported based on the alteration of the effective distance between G-quadruplex DNA and silver nanoclusters (AgNCs). Moreover, the role of the G-quadruplex structure in interacting with AgNCs has also been demonstrated. The detection of the deletion mutants of LMP1 and CCR5 genes is performed as the proof-of-concept of our assay. Finally, the potential biological application of the assay was demonstrated in 10\% v/v human serum.",

author = "Modi Wang and Wanhe Wang and Chenfu LIU and Jinbiao Liu and Kang, \{Tian Shu\} and Leung, \{Chung Hang\} and MA, \{Edmond Dik Lung\}",

note = "Funding Information: This work was supported by Hong Kong Baptist University (FRG2/14-15/004 and FRG2/15-16/002), the Health and Medical Research Fund (HMRF/14130522), the Research Grants Council (HKBU/201811, HKBU/204612 and HKBU/201913), the French Agence Nationale de la Recherche/Research Grants Council Joint Research Scheme (AHKBU201/12; Oligoswitch ANR-12-IS07-0001), National Natural Science Foundation of China (21575121), Guangdong Province Natural Science Foundation (2015A030313816), Hong Kong Baptist University Century Club Sponsorship Scheme 2015, Interdisciplinary Research Matching Scheme (RC-IRMS/14-15/06), the Science and Technology Development Fund, Macao SAR (098/2014/A2), the University of Macau (MYRG091(Y3-L2)-ICMS12-LCH and MYRG2015-00137-ICMS-QRCM).",

year = "2017",

month = jan,

doi = "10.1039/c6qm00068a",

language = "English",

volume = "1",

pages = "128--131",

journal = "Materials Chemistry Frontiers",

issn = "2052-1537",

publisher = "Royal Society of Chemistry",

number = "1",

}

TY - JOUR

T1 - A luminescence switch-on assay for the detection of specific gene deletion using G-quadruplex DNA and silver nanoclusters

AU - Wang, Modi

AU - Wang, Wanhe

AU - LIU, Chenfu

AU - Liu, Jinbiao

AU - Kang, Tian Shu

AU - Leung, Chung Hang

AU - MA, Edmond Dik Lung

N1 - Funding Information: This work was supported by Hong Kong Baptist University (FRG2/14-15/004 and FRG2/15-16/002), the Health and Medical Research Fund (HMRF/14130522), the Research Grants Council (HKBU/201811, HKBU/204612 and HKBU/201913), the French Agence Nationale de la Recherche/Research Grants Council Joint Research Scheme (AHKBU201/12; Oligoswitch ANR-12-IS07-0001), National Natural Science Foundation of China (21575121), Guangdong Province Natural Science Foundation (2015A030313816), Hong Kong Baptist University Century Club Sponsorship Scheme 2015, Interdisciplinary Research Matching Scheme (RC-IRMS/14-15/06), the Science and Technology Development Fund, Macao SAR (098/2014/A2), the University of Macau (MYRG091(Y3-L2)-ICMS12-LCH and MYRG2015-00137-ICMS-QRCM).

PY - 2017/1

Y1 - 2017/1

N2 - A switch-on gene deletion detection platform is reported based on the alteration of the effective distance between G-quadruplex DNA and silver nanoclusters (AgNCs). Moreover, the role of the G-quadruplex structure in interacting with AgNCs has also been demonstrated. The detection of the deletion mutants of LMP1 and CCR5 genes is performed as the proof-of-concept of our assay. Finally, the potential biological application of the assay was demonstrated in 10% v/v human serum.

AB - A switch-on gene deletion detection platform is reported based on the alteration of the effective distance between G-quadruplex DNA and silver nanoclusters (AgNCs). Moreover, the role of the G-quadruplex structure in interacting with AgNCs has also been demonstrated. The detection of the deletion mutants of LMP1 and CCR5 genes is performed as the proof-of-concept of our assay. Finally, the potential biological application of the assay was demonstrated in 10% v/v human serum.

UR - https://www.scopus.com/pages/publications/85042087867

U2 - 10.1039/c6qm00068a

DO - 10.1039/c6qm00068a

M3 - Journal article

AN - SCOPUS:85042087867

SN - 2052-1537

VL - 1

SP - 128

EP - 131

JO - Materials Chemistry Frontiers

JF - Materials Chemistry Frontiers

IS - 1

ER -

A luminescence switch-on assay for the detection of specific gene deletion using G-quadruplex DNA and silver nanoclusters

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