A luminescence switch-on assay for the detection of specific gene deletion using G-quadruplex DNA and silver nanoclusters

Modi Wang, Wanhe Wang, Chenfu LIU, Jinbiao Liu, Tian Shu Kang, Chung Hang Leung, Edmond Dik Lung MA*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

14 Citations (Scopus)

Abstract

A switch-on gene deletion detection platform is reported based on the alteration of the effective distance between G-quadruplex DNA and silver nanoclusters (AgNCs). Moreover, the role of the G-quadruplex structure in interacting with AgNCs has also been demonstrated. The detection of the deletion mutants of LMP1 and CCR5 genes is performed as the proof-of-concept of our assay. Finally, the potential biological application of the assay was demonstrated in 10% v/v human serum.

Original languageEnglish
Pages (from-to)128-131
Number of pages4
JournalMaterials Chemistry Frontiers
Volume1
Issue number1
DOIs
Publication statusPublished - Jan 2017

Scopus Subject Areas

  • Materials Science(all)
  • Materials Chemistry

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