A long-lived phosphorescence iridium(III) complex as a switch on-off-on probe for live zebrafish monitoring of endogenous sulfide generation

Chung Nga Ko, Chao Yang, Sheng Lin, Shengnan Li, Zhenzhen Dong, Jinbiao Liu*, Simon Ming Yuen Lee, Chung Hang Leung, Edmond Dik Lung MA

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

31 Citations (Scopus)

Abstract

In this work, we report a novel iridium(III)-based luminescent switch on-off-on probe, for the in vitro and in vivo detection of sulfide ion. The mechanism of this platform is based on the effective charge transfer quenching of the iridium(III) complex 1 by Fe3+, followed by the restoration of luminescence upon the addition of Na2S. The probe, hereinafter referred to as 1–Fe3+, exhibited a linear range of detection for Na2S from 0.01 to 1.5 mM, with a detection limit of 2.9 μM at signal-to-noise ratio (S/N) of 3. We also demonstrate the utility of 1–Fe3+ for cell-based imaging as well as for the detection of enzymatic sulfide generation in living zebrafish.

Original languageEnglish
Pages (from-to)575-583
Number of pages9
JournalBiosensors and Bioelectronics
Volume94
DOIs
Publication statusPublished - 15 Aug 2017

Scopus Subject Areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

User-Defined Keywords

  • Endogenous sulfide production
  • Iridum(III) complex
  • Sulfide detection in living organism

Fingerprint

Dive into the research topics of 'A long-lived phosphorescence iridium(III) complex as a switch on-off-on probe for live zebrafish monitoring of endogenous sulfide generation'. Together they form a unique fingerprint.

Cite this