A long-lived phosphorescence iridium(III) complex as a switch on-off-on probe for live zebrafish monitoring of endogenous sulfide generation

Chung Nga Ko; Chao Yang; Sheng Lin; Shengnan Li; Zhenzhen Dong; Jinbiao Liu; Simon Ming Yuen Lee; Chung Hang Leung; Edmond Dik Lung Ma

doi:10.1016/j.bios.2017.03.050

A long-lived phosphorescence iridium(III) complex as a switch on-off-on probe for live zebrafish monitoring of endogenous sulfide generation

Chung Nga Ko, Chao Yang, Sheng Lin, Shengnan Li, Zhenzhen Dong, Jinbiao Liu^*, Simon Ming Yuen Lee^*, Chung Hang Leung^*, Edmond Dik Lung Ma^*

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

42 Citations (Scopus)

Abstract

In this work, we report a novel iridium(III)-based luminescent switch on-off-on probe, for the in vitro and in vivo detection of sulfide ion. The mechanism of this platform is based on the effective charge transfer quenching of the iridium(III) complex 1 by Fe³⁺, followed by the restoration of luminescence upon the addition of Na₂S. The probe, hereinafter referred to as 1–Fe³⁺, exhibited a linear range of detection for Na₂S from 0.01 to 1.5 mM, with a detection limit of 2.9 μM at signal-to-noise ratio (S/N) of 3. We also demonstrate the utility of 1–Fe³⁺ for cell-based imaging as well as for the detection of enzymatic sulfide generation in living zebrafish.

Original language	English
Pages (from-to)	575-583
Number of pages	9
Journal	Biosensors and Bioelectronics
Volume	94
DOIs	https://doi.org/10.1016/j.bios.2017.03.050
Publication status	Published - 15 Aug 2017

Scopus Subject Areas

Biotechnology
Biophysics
Biomedical Engineering
Electrochemistry

User-Defined Keywords

Endogenous sulfide production
Iridum(III) complex
Sulfide detection in living organism

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10.1016/j.bios.2017.03.050

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title = "A long-lived phosphorescence iridium(III) complex as a switch on-off-on probe for live zebrafish monitoring of endogenous sulfide generation",

abstract = "In this work, we report a novel iridium(III)-based luminescent switch on-off-on probe, for the in vitro and in vivo detection of sulfide ion. The mechanism of this platform is based on the effective charge transfer quenching of the iridium(III) complex 1 by Fe3+, followed by the restoration of luminescence upon the addition of Na2S. The probe, hereinafter referred to as 1–Fe3+, exhibited a linear range of detection for Na2S from 0.01 to 1.5 mM, with a detection limit of 2.9 μM at signal-to-noise ratio (S/N) of 3. We also demonstrate the utility of 1–Fe3+ for cell-based imaging as well as for the detection of enzymatic sulfide generation in living zebrafish.",

keywords = "Endogenous sulfide production, Iridum(III) complex, Sulfide detection in living organism",

author = "Ko, {Chung Nga} and Chao Yang and Sheng Lin and Shengnan Li and Zhenzhen Dong and Jinbiao Liu and Lee, {Simon Ming Yuen} and Leung, {Chung Hang} and Ma, {Edmond Dik Lung}",

note = "Funding Information: This work is supported by the National Natural Science Foundation of China (Nos. 21502075 and 21575121), Hong Kong Baptist University (FRG2/16-17/007), the Health and Medical Research Fund (HMRF/14-15/02CHEM), the Research Grants Council (HKBU12301115), the State Key Laboratory of Environmental and Biological Analysis Research Grant (SKLP-14?15-P001), the Science and Technology Development Fund, Macao SAR (098/2014/A2) and the University of Macau (MYRG2015-00137-ICMS-QRCM, MYRG2016-00151-ICMS-QRCM and MRG044/LCH/2015/ICMS).",

year = "2017",

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doi = "10.1016/j.bios.2017.03.050",

language = "English",

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journal = "Biosensors and Bioelectronics",

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T1 - A long-lived phosphorescence iridium(III) complex as a switch on-off-on probe for live zebrafish monitoring of endogenous sulfide generation

AU - Ko, Chung Nga

AU - Yang, Chao

AU - Lin, Sheng

AU - Li, Shengnan

AU - Dong, Zhenzhen

AU - Liu, Jinbiao

AU - Lee, Simon Ming Yuen

AU - Leung, Chung Hang

AU - Ma, Edmond Dik Lung

N1 - Funding Information: This work is supported by the National Natural Science Foundation of China (Nos. 21502075 and 21575121), Hong Kong Baptist University (FRG2/16-17/007), the Health and Medical Research Fund (HMRF/14-15/02CHEM), the Research Grants Council (HKBU12301115), the State Key Laboratory of Environmental and Biological Analysis Research Grant (SKLP-14?15-P001), the Science and Technology Development Fund, Macao SAR (098/2014/A2) and the University of Macau (MYRG2015-00137-ICMS-QRCM, MYRG2016-00151-ICMS-QRCM and MRG044/LCH/2015/ICMS).

PY - 2017/8/15

Y1 - 2017/8/15

N2 - In this work, we report a novel iridium(III)-based luminescent switch on-off-on probe, for the in vitro and in vivo detection of sulfide ion. The mechanism of this platform is based on the effective charge transfer quenching of the iridium(III) complex 1 by Fe3+, followed by the restoration of luminescence upon the addition of Na2S. The probe, hereinafter referred to as 1–Fe3+, exhibited a linear range of detection for Na2S from 0.01 to 1.5 mM, with a detection limit of 2.9 μM at signal-to-noise ratio (S/N) of 3. We also demonstrate the utility of 1–Fe3+ for cell-based imaging as well as for the detection of enzymatic sulfide generation in living zebrafish.

AB - In this work, we report a novel iridium(III)-based luminescent switch on-off-on probe, for the in vitro and in vivo detection of sulfide ion. The mechanism of this platform is based on the effective charge transfer quenching of the iridium(III) complex 1 by Fe3+, followed by the restoration of luminescence upon the addition of Na2S. The probe, hereinafter referred to as 1–Fe3+, exhibited a linear range of detection for Na2S from 0.01 to 1.5 mM, with a detection limit of 2.9 μM at signal-to-noise ratio (S/N) of 3. We also demonstrate the utility of 1–Fe3+ for cell-based imaging as well as for the detection of enzymatic sulfide generation in living zebrafish.

KW - Endogenous sulfide production

KW - Iridum(III) complex

KW - Sulfide detection in living organism

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DO - 10.1016/j.bios.2017.03.050

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A long-lived phosphorescence iridium(III) complex as a switch on-off-on probe for live zebrafish monitoring of endogenous sulfide generation

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