In this work, we report a novel iridium(III)-based luminescent switch on-off-on probe, for the in vitro and in vivo detection of sulfide ion. The mechanism of this platform is based on the effective charge transfer quenching of the iridium(III) complex 1 by Fe3+, followed by the restoration of luminescence upon the addition of Na2S. The probe, hereinafter referred to as 1–Fe3+, exhibited a linear range of detection for Na2S from 0.01 to 1.5 mM, with a detection limit of 2.9 μM at signal-to-noise ratio (S/N) of 3. We also demonstrate the utility of 1–Fe3+ for cell-based imaging as well as for the detection of enzymatic sulfide generation in living zebrafish.
Scopus Subject Areas
- Biomedical Engineering
- Endogenous sulfide production
- Iridum(III) complex
- Sulfide detection in living organism