@article{6540d66a02c24d45a1d5b14dec07a35a,
title = "A long-lived peptide-conjugated iridium(iii) complex as a luminescent probe and inhibitor of the cell migration mediator, formyl peptide receptor 2",
abstract = "Formyl peptide receptors play important biological and therapeutic roles in wound repair and inflammatory diseases. In this work, we present a luminescent iridium(iii) complex (6) conjugated with the peptide agonist WKYMVm as a luminescent formyl peptide receptor 2 (FPR2) imaging probe in living cells. Complex 6 displayed ideal cell imaging characteristics, high photostability and low cytotoxicity. Competition assays with a known FPR2 antagonist, WRW4, and siRNA knockdown experiments both revealed that complex 6 selectively targeted FPR2 in living HUVEC cells. Moreover, complex 6 regulated FPR2 signalling in HUVEC cells as shown using a mechanical scratch assay. Finally, complex 6 reduced epithelial cell migration capacity and inhibited lipoxin A4 (LXA4)-triggered cell migration in HUVEC cells, demonstrating the ability of this complex to inhibit FPR2 in living cells. To our knowledge, this is the first long-lived probe for imaging FPR2 in living cells.",
author = "Kasipandi Vellaisamy and Guodong Li and Wanhe Wang and Leung, {Chung Hang} and Ma, {Edmond Dik Lung}",
note = "Funding Information: This work is supported by Hong Kong Baptist University (FRG2/ 16-17/007 and FRG2/17-18/003), the Health and Medical Research Fund (HMRF/14150561), the Research Grants Council (HKBU/12301115), the National Natural Science Foundation of China (21575121, 21775131, and 21628502), the Hong Kong Baptist University Century Club Sponsorship Scheme 2018, the Interdisciplinary Research Matching Scheme (RC-IRMS/16-17/ 03), Interdisciplinary Research Clusters Matching Scheme (RC-IRCs/17-18/03), Innovation and Technology Fund (ITS/260/ 16FX), Matching Proof of Concept Fund (MPCF-001-2017/18), Collaborative Research Fund (C5026-16G), SKLEBA and HKBU Strategic Development Fund (SKLP_1718_P04), the Science and Technology Development Fund, Macao SAR (077/2016/A2), and the University of Macau (MYRG2016-00151-ICMS-QRCM and MYRG2018-00187-ICMS).",
year = "2018",
month = nov,
day = "21",
doi = "10.1039/c8sc02733a",
language = "English",
volume = "9",
pages = "8171--8177",
journal = "Chemical Science",
issn = "2041-6520",
publisher = "Royal Society of Chemistry",
number = "43",
}