A light sheet based high throughput 3D-imaging flow cytometer for phytoplankton analysis

Jianglai Wu; Jianping Li; Robert K.Y. Chan

doi:10.1364/OE.21.014474

A light sheet based high throughput 3D-imaging flow cytometer for phytoplankton analysis

Jianglai Wu, Jianping Li, Robert K.Y. Chan

Department of Physics

Research output: Contribution to journal › Journal article › peer-review

79 Citations (Scopus)

Abstract

This paper reports a light sheet fluorescence imaging flow cytometer for 3D sectioning of phytoplankton. The instrument developed has the inherent advantages of high cell counting throughput and high spatial resolution information derived from flow cytometry and light sheet microscopy. The throughput of the instrument is quantified by the sample volume flow rate of 0.5 μl/min with a spatial resolution as achieved by light sheet microscopy. Preliminary results from 3D morphology of the internal chlorophyll-a structure of two dinoflagellates species show promising application potentials of the method for phytoplankton taxonomy of selected species and species groups.

Original language	English
Pages (from-to)	14474-14480
Number of pages	7
Journal	Optics Express
Volume	21
Issue number	12
DOIs	https://doi.org/10.1364/OE.21.014474
Publication status	Published - Jun 2013

Scopus Subject Areas

Atomic and Molecular Physics, and Optics

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10.1364/OE.21.014474

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title = "A light sheet based high throughput 3D-imaging flow cytometer for phytoplankton analysis",

abstract = "This paper reports a light sheet fluorescence imaging flow cytometer for 3D sectioning of phytoplankton. The instrument developed has the inherent advantages of high cell counting throughput and high spatial resolution information derived from flow cytometry and light sheet microscopy. The throughput of the instrument is quantified by the sample volume flow rate of 0.5 μl/min with a spatial resolution as achieved by light sheet microscopy. Preliminary results from 3D morphology of the internal chlorophyll-a structure of two dinoflagellates species show promising application potentials of the method for phytoplankton taxonomy of selected species and species groups.",

author = "Jianglai Wu and Jianping Li and Chan, {Robert K.Y.}",

note = "We gratefully acknowledge the financial support from State Key Laboratory in Marine Pollution (SKLMP) of City University of Hong Kong (Major National Science and Technology Programs “Integrated Technology Development for Algal Bloom Online Monitoring and Validations in Lake Tai”) and from Hong Kong Baptist University (FRG2/11-12/092 and IRACE/11-12/05). ",

year = "2013",

month = jun,

doi = "10.1364/OE.21.014474",

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AU - Li, Jianping

AU - Chan, Robert K.Y.

N1 - We gratefully acknowledge the financial support from State Key Laboratory in Marine Pollution (SKLMP) of City University of Hong Kong (Major National Science and Technology Programs “Integrated Technology Development for Algal Bloom Online Monitoring and Validations in Lake Tai”) and from Hong Kong Baptist University (FRG2/11-12/092 and IRACE/11-12/05).

PY - 2013/6

Y1 - 2013/6

N2 - This paper reports a light sheet fluorescence imaging flow cytometer for 3D sectioning of phytoplankton. The instrument developed has the inherent advantages of high cell counting throughput and high spatial resolution information derived from flow cytometry and light sheet microscopy. The throughput of the instrument is quantified by the sample volume flow rate of 0.5 μl/min with a spatial resolution as achieved by light sheet microscopy. Preliminary results from 3D morphology of the internal chlorophyll-a structure of two dinoflagellates species show promising application potentials of the method for phytoplankton taxonomy of selected species and species groups.

AB - This paper reports a light sheet fluorescence imaging flow cytometer for 3D sectioning of phytoplankton. The instrument developed has the inherent advantages of high cell counting throughput and high spatial resolution information derived from flow cytometry and light sheet microscopy. The throughput of the instrument is quantified by the sample volume flow rate of 0.5 μl/min with a spatial resolution as achieved by light sheet microscopy. Preliminary results from 3D morphology of the internal chlorophyll-a structure of two dinoflagellates species show promising application potentials of the method for phytoplankton taxonomy of selected species and species groups.

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A light sheet based high throughput 3D-imaging flow cytometer for phytoplankton analysis

Abstract

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