A light sheet based high throughput 3D-imaging flow cytometer for phytoplankton analysis

Jianglai Wu, Jianping LI, Robert K Y CHAN

Research output: Contribution to journalArticlepeer-review

54 Citations (Scopus)

Abstract

This paper reports a light sheet fluorescence imaging flow cytometer for 3D sectioning of phytoplankton. The instrument developed has the inherent advantages of high cell counting throughput and high spatial resolution information derived from flow cytometry and light sheet microscopy. The throughput of the instrument is quantified by the sample volume flow rate of 0.5 μl/min with a spatial resolution as achieved by light sheet microscopy. Preliminary results from 3D morphology of the internal chlorophyll-a structure of two dinoflagellates species show promising application potentials of the method for phytoplankton taxonomy of selected species and species groups.

Original languageEnglish
Pages (from-to)14474-14480
Number of pages7
JournalOptics Express
Volume21
Issue number12
DOIs
Publication statusPublished - Jun 2013

Scopus Subject Areas

  • Atomic and Molecular Physics, and Optics

Fingerprint

Dive into the research topics of 'A light sheet based high throughput 3D-imaging flow cytometer for phytoplankton analysis'. Together they form a unique fingerprint.

Cite this