A Label-Free Luminescent Switch-On Assay for ATP Using a G-Quadruplex-Selective Iridium(III) Complex

Ka Ho Leung; Lihua Lu; Modi Wang; Tsun Yin Mak; Daniel Shiu Hin Chan; Fung Kit Tang; Chung Hang Leung; Hiu Yee Kwan; Zhiling YU; Edmond Dik Lung MA

doi:10.1371/journal.pone.0077021

A Label-Free Luminescent Switch-On Assay for ATP Using a G-Quadruplex-Selective Iridium(III) Complex

Ka Ho Leung
, Lihua Lu
, Modi Wang
, Tsun Yin Mak
, Daniel Shiu Hin Chan
, Fung Kit Tang
, Chung Hang Leung
, Hiu Yee Kwan
, Zhiling YU
, Edmond Dik Lung MA^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › peer-review

20 Citations (Scopus)

Abstract

We report herein the G-quadruplex-selective property of a luminescent cyclometallated iridium(III) complex for the detection of adenosine-5′-triphosphate (ATP) in aqueous solution. The ATP-binding aptamer was employed as the ATP recognition unit, while the iridium(III) complex was used to monitor the formation of the G-quadruplex structure induced by ATP. The sensitivity and fold enhancement of the assay were higher than those of the previously reported assay using the organic dye crystal violet as a fluorescent probe. This label-free luminescent switch-on assay exhibits high sensitivity and selectivity towards ATP with a limit of detection of 2.5 μM.

Original language	English
Article number	e77021
Journal	PLoS ONE
Volume	8
Issue number	10
DOIs	https://doi.org/10.1371/journal.pone.0077021
Publication status	Published - 25 Oct 2013

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title = "A Label-Free Luminescent Switch-On Assay for ATP Using a G-Quadruplex-Selective Iridium(III) Complex",

abstract = "We report herein the G-quadruplex-selective property of a luminescent cyclometallated iridium(III) complex for the detection of adenosine-5′-triphosphate (ATP) in aqueous solution. The ATP-binding aptamer was employed as the ATP recognition unit, while the iridium(III) complex was used to monitor the formation of the G-quadruplex structure induced by ATP. The sensitivity and fold enhancement of the assay were higher than those of the previously reported assay using the organic dye crystal violet as a fluorescent probe. This label-free luminescent switch-on assay exhibits high sensitivity and selectivity towards ATP with a limit of detection of 2.5 μM.",

author = "Leung, \{Ka Ho\} and Lihua Lu and Modi Wang and Mak, \{Tsun Yin\} and Chan, \{Daniel Shiu Hin\} and Tang, \{Fung Kit\} and Leung, \{Chung Hang\} and Kwan, \{Hiu Yee\} and Zhiling YU and MA, \{Edmond Dik Lung\}",

note = "This work is supported by Hong Kong Baptist University (FRG2/11-12/009, FRG2/12-13/021), Centre for Cancer and Inflammation Research, School of Chinese Medicine (CCIR-SCM, HKBU), the Health and Medical Research Fund (HMRF/11101212), the Research Grants Council (HKBU/201811 and HKBU/204612), the French National Research Agency/Research Grants Council Joint Research Scheme (A-HKBU201/12), the Science and Technology Development Fund, Macao SAR (001/2012/A) and the University of Macau (MYRG091(Y2-L2)-ICMS12-LCH and MYRG121(Y2-L2)-ICMS12-LCH). ",

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doi = "10.1371/journal.pone.0077021",

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T1 - A Label-Free Luminescent Switch-On Assay for ATP Using a G-Quadruplex-Selective Iridium(III) Complex

AU - Leung, Ka Ho

AU - Lu, Lihua

AU - Wang, Modi

AU - Mak, Tsun Yin

AU - Chan, Daniel Shiu Hin

AU - Tang, Fung Kit

AU - Leung, Chung Hang

AU - Kwan, Hiu Yee

AU - YU, Zhiling

AU - MA, Edmond Dik Lung

N1 - This work is supported by Hong Kong Baptist University (FRG2/11-12/009, FRG2/12-13/021), Centre for Cancer and Inflammation Research, School of Chinese Medicine (CCIR-SCM, HKBU), the Health and Medical Research Fund (HMRF/11101212), the Research Grants Council (HKBU/201811 and HKBU/204612), the French National Research Agency/Research Grants Council Joint Research Scheme (A-HKBU201/12), the Science and Technology Development Fund, Macao SAR (001/2012/A) and the University of Macau (MYRG091(Y2-L2)-ICMS12-LCH and MYRG121(Y2-L2)-ICMS12-LCH).

PY - 2013/10/25

Y1 - 2013/10/25

N2 - We report herein the G-quadruplex-selective property of a luminescent cyclometallated iridium(III) complex for the detection of adenosine-5′-triphosphate (ATP) in aqueous solution. The ATP-binding aptamer was employed as the ATP recognition unit, while the iridium(III) complex was used to monitor the formation of the G-quadruplex structure induced by ATP. The sensitivity and fold enhancement of the assay were higher than those of the previously reported assay using the organic dye crystal violet as a fluorescent probe. This label-free luminescent switch-on assay exhibits high sensitivity and selectivity towards ATP with a limit of detection of 2.5 μM.

AB - We report herein the G-quadruplex-selective property of a luminescent cyclometallated iridium(III) complex for the detection of adenosine-5′-triphosphate (ATP) in aqueous solution. The ATP-binding aptamer was employed as the ATP recognition unit, while the iridium(III) complex was used to monitor the formation of the G-quadruplex structure induced by ATP. The sensitivity and fold enhancement of the assay were higher than those of the previously reported assay using the organic dye crystal violet as a fluorescent probe. This label-free luminescent switch-on assay exhibits high sensitivity and selectivity towards ATP with a limit of detection of 2.5 μM.

UR - https://www.scopus.com/pages/publications/84886446398

U2 - 10.1371/journal.pone.0077021

DO - 10.1371/journal.pone.0077021

M3 - Journal article

C2 - 24204723

AN - SCOPUS:84886446398

SN - 1932-6203

VL - 8

JO - PLoS ONE

JF - PLoS ONE

IS - 10

M1 - e77021

ER -

A Label-Free Luminescent Switch-On Assay for ATP Using a G-Quadruplex-Selective Iridium(III) Complex

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