A Label-Free Luminescent Switch-On Assay for ATP Using a G-Quadruplex-Selective Iridium(III) Complex

Ka Ho Leung; Lihua Lu; Modi Wang; Tsun Yin Mak; Daniel Shiu Hin Chan; Fung Kit Tang; Chung Hang Leung; Hiu Yee Kwan; Zhiling YU; Edmond Dik Lung MA

doi:10.1371/journal.pone.0077021

A Label-Free Luminescent Switch-On Assay for ATP Using a G-Quadruplex-Selective Iridium(III) Complex

Ka Ho Leung, Lihua Lu, Modi Wang, Tsun Yin Mak, Daniel Shiu Hin Chan, Fung Kit Tang, Chung Hang Leung, Hiu Yee Kwan, Zhiling YU, Edmond Dik Lung MA^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › peer-review

19 Citations (Scopus)

Abstract

We report herein the G-quadruplex-selective property of a luminescent cyclometallated iridium(III) complex for the detection of adenosine-5′-triphosphate (ATP) in aqueous solution. The ATP-binding aptamer was employed as the ATP recognition unit, while the iridium(III) complex was used to monitor the formation of the G-quadruplex structure induced by ATP. The sensitivity and fold enhancement of the assay were higher than those of the previously reported assay using the organic dye crystal violet as a fluorescent probe. This label-free luminescent switch-on assay exhibits high sensitivity and selectivity towards ATP with a limit of detection of 2.5 μM.

Original language	English
Article number	e77021
Journal	PLoS ONE
Volume	8
Issue number	10
DOIs	https://doi.org/10.1371/journal.pone.0077021
Publication status	Published - 25 Oct 2013

Scopus Subject Areas

Biochemistry, Genetics and Molecular Biology(all)
Agricultural and Biological Sciences(all)
General

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title = "A Label-Free Luminescent Switch-On Assay for ATP Using a G-Quadruplex-Selective Iridium(III) Complex",

abstract = "We report herein the G-quadruplex-selective property of a luminescent cyclometallated iridium(III) complex for the detection of adenosine-5′-triphosphate (ATP) in aqueous solution. The ATP-binding aptamer was employed as the ATP recognition unit, while the iridium(III) complex was used to monitor the formation of the G-quadruplex structure induced by ATP. The sensitivity and fold enhancement of the assay were higher than those of the previously reported assay using the organic dye crystal violet as a fluorescent probe. This label-free luminescent switch-on assay exhibits high sensitivity and selectivity towards ATP with a limit of detection of 2.5 μM.",

author = "Leung, {Ka Ho} and Lihua Lu and Modi Wang and Mak, {Tsun Yin} and Chan, {Daniel Shiu Hin} and Tang, {Fung Kit} and Leung, {Chung Hang} and Kwan, {Hiu Yee} and Zhiling YU and MA, {Edmond Dik Lung}",

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T1 - A Label-Free Luminescent Switch-On Assay for ATP Using a G-Quadruplex-Selective Iridium(III) Complex

AU - Leung, Ka Ho

AU - Lu, Lihua

AU - Wang, Modi

AU - Mak, Tsun Yin

AU - Chan, Daniel Shiu Hin

AU - Tang, Fung Kit

AU - Leung, Chung Hang

AU - Kwan, Hiu Yee

AU - YU, Zhiling

AU - MA, Edmond Dik Lung

PY - 2013/10/25

Y1 - 2013/10/25

N2 - We report herein the G-quadruplex-selective property of a luminescent cyclometallated iridium(III) complex for the detection of adenosine-5′-triphosphate (ATP) in aqueous solution. The ATP-binding aptamer was employed as the ATP recognition unit, while the iridium(III) complex was used to monitor the formation of the G-quadruplex structure induced by ATP. The sensitivity and fold enhancement of the assay were higher than those of the previously reported assay using the organic dye crystal violet as a fluorescent probe. This label-free luminescent switch-on assay exhibits high sensitivity and selectivity towards ATP with a limit of detection of 2.5 μM.

AB - We report herein the G-quadruplex-selective property of a luminescent cyclometallated iridium(III) complex for the detection of adenosine-5′-triphosphate (ATP) in aqueous solution. The ATP-binding aptamer was employed as the ATP recognition unit, while the iridium(III) complex was used to monitor the formation of the G-quadruplex structure induced by ATP. The sensitivity and fold enhancement of the assay were higher than those of the previously reported assay using the organic dye crystal violet as a fluorescent probe. This label-free luminescent switch-on assay exhibits high sensitivity and selectivity towards ATP with a limit of detection of 2.5 μM.

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U2 - 10.1371/journal.pone.0077021

DO - 10.1371/journal.pone.0077021

M3 - Journal article

C2 - 24204723

AN - SCOPUS:84886446398

SN - 1932-6203

VL - 8

JO - PLoS ONE

JF - PLoS ONE

IS - 10

M1 - e77021

ER -

A Label-Free Luminescent Switch-On Assay for ATP Using a G-Quadruplex-Selective Iridium(III) Complex

Abstract

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