A label-free G-quadruplex-based mercury detection assay employing the exonuclease III-mediated cleavage of T-Hg2+-T mismatched DNA

Wanhe Wang, Tian Shu Kang, Philip Wai Hong Chan, Jin Jian Lu, Xiu Ping Chen, Chung Hang Leung, Edmond Dik Lung MA

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

We report herein the use of an exonuclease III and G-quadruplex probe to construct a G-quadruplex-based luminescence detection platform for Hg2+. Unlike common DNA-based Hg2+ detection methods, when using the dsDNA probe to monitor the hairpin formation, the intercalation of the dsDNA probe may be influenced by the distortion of dsDNA. This 'mix-and-detect' methodology utilized the G-quadruplex probe as the signal transducer and is simple, rapid, convenient to use and can detect down to 20 nM of Hg2+.

Original languageEnglish
Article number065004
JournalScience and Technology of Advanced Materials
Volume16
Issue number6
DOIs
Publication statusPublished - 17 Nov 2015

Scopus Subject Areas

  • Materials Science(all)

User-Defined Keywords

  • exonuclease III
  • G-quadruplex
  • iridium(III)
  • mercury(II) ion

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