A label-free G-quadruplex-based mercury detection assay employing the exonuclease III-mediated cleavage of T-Hg2+-T mismatched DNA

Wanhe Wang; Tian Shu Kang; Philip Wai Hong Chan; Jin Jian Lu; Xiu Ping Chen; Chung Hang Leung; Edmond Dik Lung MA

doi:10.1088/1468-6996/16/6/065004

A label-free G-quadruplex-based mercury detection assay employing the exonuclease III-mediated cleavage of T-Hg²⁺-T mismatched DNA

Wanhe Wang, Tian Shu Kang, Philip Wai Hong Chan, Jin Jian Lu, Xiu Ping Chen, Chung Hang Leung, Edmond Dik Lung MA

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

3 Downloads (Pure)

Abstract

We report herein the use of an exonuclease III and G-quadruplex probe to construct a G-quadruplex-based luminescence detection platform for Hg²⁺. Unlike common DNA-based Hg²⁺ detection methods, when using the dsDNA probe to monitor the hairpin formation, the intercalation of the dsDNA probe may be influenced by the distortion of dsDNA. This 'mix-and-detect' methodology utilized the G-quadruplex probe as the signal transducer and is simple, rapid, convenient to use and can detect down to 20 nM of Hg²⁺.

Original language	English
Article number	065004
Journal	Science and Technology of Advanced Materials
Volume	16
Issue number	6
DOIs	https://doi.org/10.1088/1468-6996/16/6/065004
Publication status	Published - 17 Nov 2015

Scopus Subject Areas

Materials Science(all)

User-Defined Keywords

exonuclease III
G-quadruplex
iridium(III)
mercury(II) ion

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10.1088/1468-6996/16/6/065004

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Cite this

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abstract = "We report herein the use of an exonuclease III and G-quadruplex probe to construct a G-quadruplex-based luminescence detection platform for Hg2+. Unlike common DNA-based Hg2+ detection methods, when using the dsDNA probe to monitor the hairpin formation, the intercalation of the dsDNA probe may be influenced by the distortion of dsDNA. This 'mix-and-detect' methodology utilized the G-quadruplex probe as the signal transducer and is simple, rapid, convenient to use and can detect down to 20 nM of Hg2+.",

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AU - Lu, Jin Jian

AU - Chen, Xiu Ping

AU - Leung, Chung Hang

AU - MA, Edmond Dik Lung

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AB - We report herein the use of an exonuclease III and G-quadruplex probe to construct a G-quadruplex-based luminescence detection platform for Hg2+. Unlike common DNA-based Hg2+ detection methods, when using the dsDNA probe to monitor the hairpin formation, the intercalation of the dsDNA probe may be influenced by the distortion of dsDNA. This 'mix-and-detect' methodology utilized the G-quadruplex probe as the signal transducer and is simple, rapid, convenient to use and can detect down to 20 nM of Hg2+.

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KW - iridium(III)

KW - mercury(II) ion

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