A label-free G-quadruplex-based luminescent switch-on assay for the selective detection of histidine

Hong Zhang He; Modi Wang; Daniel Shiu Hin Chan; Chung Hang Leung; Jian Wen Qiu; Dik Lung Ma

doi:10.1016/j.ymeth.2013.07.023

A label-free G-quadruplex-based luminescent switch-on assay for the selective detection of histidine

Hong Zhang He, Modi Wang, Daniel Shiu Hin Chan, Chung Hang Leung, Jian Wen Qiu, Dik Lung Ma^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › peer-review

28 Citations (Scopus)

Abstract

A label-free G-quadruplex-based luminescent switch-on assay has been developed for the selective detection of micromolar histidine in aqueous solution. In this study, an iridium(III) complex was employed as a G-quadruplex-specific luminescent probe while a guanine-rich oligonucleotide (Pu27, 5'-TG₄AG₃TG₄AG₃TG₄A₂G₂-3')/cupric ion (Cu²⁺) ensemble was employed as a recognition unit for histidine. The initial luminescence of the iridium(III) complex in the presence of G-quadruplex DNA is effectively quenched by Cu²⁺ ions due to the Cu²⁺-mediated unfolding of the G-quadruplex motif. The addition of histidine sequesters Cu²⁺ ions from the ensemble, thereby restoring the luminescence of the system. The assay could detect down to 1μM of histidine in aqueous media, and also exhibited good selectivity for histidine over other amino acids with the use of the cysteine, masking agent N-ethylmaleimide. Furthermore, the application of the assay for the detection of histidine in diluted urine samples was demonstrated.

Original language	English
Pages (from-to)	205-211
Number of pages	7
Journal	Methods
Volume	64
Issue number	3
DOIs	https://doi.org/10.1016/j.ymeth.2013.07.023
Publication status	Published - 15 Dec 2013

Scopus Subject Areas

Molecular Biology
General Biochemistry,Genetics and Molecular Biology

User-Defined Keywords

G-quadruplex
Histidine
Iridium(III) complex
Label-free
Luminescence

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10.1016/j.ymeth.2013.07.023

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@article{0202a13108a24e9fb28e681ba0be0068,

title = "A label-free G-quadruplex-based luminescent switch-on assay for the selective detection of histidine",

abstract = "A label-free G-quadruplex-based luminescent switch-on assay has been developed for the selective detection of micromolar histidine in aqueous solution. In this study, an iridium(III) complex was employed as a G-quadruplex-specific luminescent probe while a guanine-rich oligonucleotide (Pu27, 5'-TG4AG3TG4AG3TG4A2G2-3')/cupric ion (Cu2+) ensemble was employed as a recognition unit for histidine. The initial luminescence of the iridium(III) complex in the presence of G-quadruplex DNA is effectively quenched by Cu2+ ions due to the Cu2+-mediated unfolding of the G-quadruplex motif. The addition of histidine sequesters Cu2+ ions from the ensemble, thereby restoring the luminescence of the system. The assay could detect down to 1μM of histidine in aqueous media, and also exhibited good selectivity for histidine over other amino acids with the use of the cysteine, masking agent N-ethylmaleimide. Furthermore, the application of the assay for the detection of histidine in diluted urine samples was demonstrated.",

keywords = "G-quadruplex, Histidine, Iridium(III) complex, Label-free, Luminescence",

author = "He, {Hong Zhang} and Modi Wang and Chan, {Daniel Shiu Hin} and Leung, {Chung Hang} and Qiu, {Jian Wen} and Ma, {Dik Lung}",

note = "Funding Information: This work is supported by Hong Kong Baptist University ( FRG2/11-12/009 and FRG2/12-13/021 ), Centre for Cancer and Inflammation Research, School of Chinese Medicine (CCIR-SCM, HKBU), the Health and Medical Research Fund ( HMRF/11101212 ), the Research Grants Council ( HKBU/201811 , HKBU/204612 and HKBU/201913 ), the French National Research Agency/Research Grants Council Joint Research Scheme ( A-HKBU201/12 ),the Science and Technology Development Fund, Macao SAR ( 001/2012/A ) and the University of Macau ( MYRG091(Y2-L2)-ICMS12-LCH and MYRG121(Y2-L2)-ICMS12-LCH ). ",

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AU - He, Hong Zhang

AU - Wang, Modi

AU - Chan, Daniel Shiu Hin

AU - Leung, Chung Hang

AU - Qiu, Jian Wen

AU - Ma, Dik Lung

N1 - Funding Information: This work is supported by Hong Kong Baptist University ( FRG2/11-12/009 and FRG2/12-13/021 ), Centre for Cancer and Inflammation Research, School of Chinese Medicine (CCIR-SCM, HKBU), the Health and Medical Research Fund ( HMRF/11101212 ), the Research Grants Council ( HKBU/201811 , HKBU/204612 and HKBU/201913 ), the French National Research Agency/Research Grants Council Joint Research Scheme ( A-HKBU201/12 ),the Science and Technology Development Fund, Macao SAR ( 001/2012/A ) and the University of Macau ( MYRG091(Y2-L2)-ICMS12-LCH and MYRG121(Y2-L2)-ICMS12-LCH ).

PY - 2013/12/15

Y1 - 2013/12/15

N2 - A label-free G-quadruplex-based luminescent switch-on assay has been developed for the selective detection of micromolar histidine in aqueous solution. In this study, an iridium(III) complex was employed as a G-quadruplex-specific luminescent probe while a guanine-rich oligonucleotide (Pu27, 5'-TG4AG3TG4AG3TG4A2G2-3')/cupric ion (Cu2+) ensemble was employed as a recognition unit for histidine. The initial luminescence of the iridium(III) complex in the presence of G-quadruplex DNA is effectively quenched by Cu2+ ions due to the Cu2+-mediated unfolding of the G-quadruplex motif. The addition of histidine sequesters Cu2+ ions from the ensemble, thereby restoring the luminescence of the system. The assay could detect down to 1μM of histidine in aqueous media, and also exhibited good selectivity for histidine over other amino acids with the use of the cysteine, masking agent N-ethylmaleimide. Furthermore, the application of the assay for the detection of histidine in diluted urine samples was demonstrated.

AB - A label-free G-quadruplex-based luminescent switch-on assay has been developed for the selective detection of micromolar histidine in aqueous solution. In this study, an iridium(III) complex was employed as a G-quadruplex-specific luminescent probe while a guanine-rich oligonucleotide (Pu27, 5'-TG4AG3TG4AG3TG4A2G2-3')/cupric ion (Cu2+) ensemble was employed as a recognition unit for histidine. The initial luminescence of the iridium(III) complex in the presence of G-quadruplex DNA is effectively quenched by Cu2+ ions due to the Cu2+-mediated unfolding of the G-quadruplex motif. The addition of histidine sequesters Cu2+ ions from the ensemble, thereby restoring the luminescence of the system. The assay could detect down to 1μM of histidine in aqueous media, and also exhibited good selectivity for histidine over other amino acids with the use of the cysteine, masking agent N-ethylmaleimide. Furthermore, the application of the assay for the detection of histidine in diluted urine samples was demonstrated.

KW - G-quadruplex

KW - Histidine

KW - Iridium(III) complex

KW - Label-free

KW - Luminescence

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DO - 10.1016/j.ymeth.2013.07.023

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AN - SCOPUS:84888210678

SN - 1046-2023

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SP - 205

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JO - Methods

JF - Methods

IS - 3

ER -

A label-free G-quadruplex-based luminescent switch-on assay for the selective detection of histidine

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