TY - JOUR
T1 - A hybrid monolithic column based on boronate-functionalized graphene oxide nanosheets for online specific enrichment of glycoproteins
AU - Zhou, Chanyuan
AU - Chen, Xiaoman
AU - Du, Zhuo
AU - Li, Gongke
AU - Xiao, Xiaohua
AU - CAI, Zongwei
N1 - Funding Information:
The work were supported by the National Natural Science Foundation of China (Nos. 21475153, 21675178, 21375155 and 21675179), the Guangdong Provincial Natural Science Foundation of China (No. 2015A030311020), the Special Funds for Public Welfare Research and Capacity Building in Guangdong Province of China (No. 2015A030401036), and the Guangzhou Science and Technology Program of China (No. 201604020165), respectively.
PY - 2017/5/19
Y1 - 2017/5/19
N2 - A hybrid monolithic column based on aminophenylboronic acid (APBA)-functionalized graphene oxide (GO) has been developed and used for selective enrichment of glycoproteins. The APBA/GO composites were homogeneously incorporated into a polymer monolithic column with the help of oligomer matrix and followed by in situ polymerization. The effect of dispersion of APBA/GO composites in the polymerization mixture on the performance of the monolithic column was explored in detail. The presence of graphene oxide not only enlarged the BET surface area from 6.3 m2/g to 169.4 m2/g, but also provided abundant boronic acid moieties for glycoprotein extraction, which improved the enrichment selectivity and efficiency for glycoproteins. The APBA/GO hybrid monolithic column was incorporated into a sequential injection system, which facilitated online extraction of proteins. Combining the superior properties of extraordinary surface area of GO and the affinity interaction of APBA to glycoproteins, the APBA/GO hybrid monolithic column showed higher enrichment factors for glycoproteins than other proteins without cis-diol-containing groups. Also, under comparable or even shorter processing time and without the addition of any organic solvent, it showed higher binding capacity toward glycoproteins compared with the conventional boronate affinity monolithic column. The practical applicability of this system was demonstrated by processing of egg white samples for extraction of ovalbumin and ovotransferrin, and satisfactory results were obtained by assay with SDS-PAGE.
AB - A hybrid monolithic column based on aminophenylboronic acid (APBA)-functionalized graphene oxide (GO) has been developed and used for selective enrichment of glycoproteins. The APBA/GO composites were homogeneously incorporated into a polymer monolithic column with the help of oligomer matrix and followed by in situ polymerization. The effect of dispersion of APBA/GO composites in the polymerization mixture on the performance of the monolithic column was explored in detail. The presence of graphene oxide not only enlarged the BET surface area from 6.3 m2/g to 169.4 m2/g, but also provided abundant boronic acid moieties for glycoprotein extraction, which improved the enrichment selectivity and efficiency for glycoproteins. The APBA/GO hybrid monolithic column was incorporated into a sequential injection system, which facilitated online extraction of proteins. Combining the superior properties of extraordinary surface area of GO and the affinity interaction of APBA to glycoproteins, the APBA/GO hybrid monolithic column showed higher enrichment factors for glycoproteins than other proteins without cis-diol-containing groups. Also, under comparable or even shorter processing time and without the addition of any organic solvent, it showed higher binding capacity toward glycoproteins compared with the conventional boronate affinity monolithic column. The practical applicability of this system was demonstrated by processing of egg white samples for extraction of ovalbumin and ovotransferrin, and satisfactory results were obtained by assay with SDS-PAGE.
KW - Boronate affinity
KW - Glycoproteins
KW - Graphene oxide nanosheets
KW - Hybrid monolithic column
KW - On-line enrichment
UR - http://www.scopus.com/inward/record.url?scp=85010619117&partnerID=8YFLogxK
U2 - 10.1016/j.chroma.2017.01.049
DO - 10.1016/j.chroma.2017.01.049
M3 - Journal article
C2 - 28131590
AN - SCOPUS:85010619117
SN - 0021-9673
VL - 1498
SP - 90
EP - 98
JO - Journal of Chromatography A
JF - Journal of Chromatography A
ER -