TY - JOUR
T1 - A homocysteine biosensor with eggshell membrane as an enzyme immobilization platform
AU - Zhang, Guomei
AU - Liu, Diansheng
AU - Shuang, Shaomin
AU - Choi, Martin M.F.
N1 - Funding Information:
The work described in this paper was supported by a faculty research grant of Hong Kong Baptist University (FRG/01-02/II-08).
PY - 2006/4/26
Y1 - 2006/4/26
N2 - A low-cost and simple biosensing method for screening homocysteine in human plasma has been developed. The biosensor was fabricated from a d-amino acid oxidase immobilized eggshell membrane and an oxygen electrode. The effects of enzyme loading, glutaraldehyde concentration, pH, ionic strength, and temperature on the response of the biosensor have been investigated in detail. The sensing method is based on monitoring the decrease in the dissolved oxygen level of the sample solution. The linear working range was 0.05-1.5 mM and the limit of detection was 30 μM. The proposed biosensor shows fast response time (<1 min), good repeatability (n = 6, relative standard deviation = 2.38%), and long-term stability (85% of its initial sensitivity after 2 months of storage).
AB - A low-cost and simple biosensing method for screening homocysteine in human plasma has been developed. The biosensor was fabricated from a d-amino acid oxidase immobilized eggshell membrane and an oxygen electrode. The effects of enzyme loading, glutaraldehyde concentration, pH, ionic strength, and temperature on the response of the biosensor have been investigated in detail. The sensing method is based on monitoring the decrease in the dissolved oxygen level of the sample solution. The linear working range was 0.05-1.5 mM and the limit of detection was 30 μM. The proposed biosensor shows fast response time (<1 min), good repeatability (n = 6, relative standard deviation = 2.38%), and long-term stability (85% of its initial sensitivity after 2 months of storage).
KW - d-Amino acid oxidase
KW - Eggshell membrane
KW - Homocysteine biosensor
UR - http://www.scopus.com/inward/record.url?scp=33645151981&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2005.08.011
DO - 10.1016/j.snb.2005.08.011
M3 - Journal article
AN - SCOPUS:33645151981
SN - 0925-4005
VL - 114
SP - 936
EP - 942
JO - Sensors and Actuators B: Chemical
JF - Sensors and Actuators B: Chemical
IS - 2
ER -