A homocysteine biosensor with eggshell membrane as an enzyme immobilization platform

Guomei Zhang; Diansheng Liu; Shaomin Shuang; Martin M F CHOI

doi:10.1016/j.snb.2005.08.011

A homocysteine biosensor with eggshell membrane as an enzyme immobilization platform

Guomei Zhang, Diansheng Liu, Shaomin Shuang, Martin M F CHOI^*

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

59 Citations (Scopus)

Abstract

A low-cost and simple biosensing method for screening homocysteine in human plasma has been developed. The biosensor was fabricated from a d-amino acid oxidase immobilized eggshell membrane and an oxygen electrode. The effects of enzyme loading, glutaraldehyde concentration, pH, ionic strength, and temperature on the response of the biosensor have been investigated in detail. The sensing method is based on monitoring the decrease in the dissolved oxygen level of the sample solution. The linear working range was 0.05-1.5 mM and the limit of detection was 30 μM. The proposed biosensor shows fast response time (<1 min), good repeatability (n = 6, relative standard deviation = 2.38%), and long-term stability (85% of its initial sensitivity after 2 months of storage).

Original language	English
Pages (from-to)	936-942
Number of pages	7
Journal	Sensors and Actuators, B: Chemical
Volume	114
Issue number	2
DOIs	https://doi.org/10.1016/j.snb.2005.08.011
Publication status	Published - 26 Apr 2006

Scopus Subject Areas

Electronic, Optical and Magnetic Materials
Instrumentation
Condensed Matter Physics
Surfaces, Coatings and Films
Metals and Alloys
Electrical and Electronic Engineering
Materials Chemistry

User-Defined Keywords

d-Amino acid oxidase
Eggshell membrane
Homocysteine biosensor

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10.1016/j.snb.2005.08.011

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title = "A homocysteine biosensor with eggshell membrane as an enzyme immobilization platform",

abstract = "A low-cost and simple biosensing method for screening homocysteine in human plasma has been developed. The biosensor was fabricated from a d-amino acid oxidase immobilized eggshell membrane and an oxygen electrode. The effects of enzyme loading, glutaraldehyde concentration, pH, ionic strength, and temperature on the response of the biosensor have been investigated in detail. The sensing method is based on monitoring the decrease in the dissolved oxygen level of the sample solution. The linear working range was 0.05-1.5 mM and the limit of detection was 30 μM. The proposed biosensor shows fast response time (<1 min), good repeatability (n = 6, relative standard deviation = 2.38%), and long-term stability (85% of its initial sensitivity after 2 months of storage).",

keywords = "d-Amino acid oxidase, Eggshell membrane, Homocysteine biosensor",

author = "Guomei Zhang and Diansheng Liu and Shaomin Shuang and CHOI, {Martin M F}",

note = "Funding Information: The work described in this paper was supported by a faculty research grant of Hong Kong Baptist University (FRG/01-02/II-08). ",

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T1 - A homocysteine biosensor with eggshell membrane as an enzyme immobilization platform

AU - Zhang, Guomei

AU - Liu, Diansheng

AU - Shuang, Shaomin

AU - CHOI, Martin M F

N1 - Funding Information: The work described in this paper was supported by a faculty research grant of Hong Kong Baptist University (FRG/01-02/II-08).

PY - 2006/4/26

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N2 - A low-cost and simple biosensing method for screening homocysteine in human plasma has been developed. The biosensor was fabricated from a d-amino acid oxidase immobilized eggshell membrane and an oxygen electrode. The effects of enzyme loading, glutaraldehyde concentration, pH, ionic strength, and temperature on the response of the biosensor have been investigated in detail. The sensing method is based on monitoring the decrease in the dissolved oxygen level of the sample solution. The linear working range was 0.05-1.5 mM and the limit of detection was 30 μM. The proposed biosensor shows fast response time (<1 min), good repeatability (n = 6, relative standard deviation = 2.38%), and long-term stability (85% of its initial sensitivity after 2 months of storage).

AB - A low-cost and simple biosensing method for screening homocysteine in human plasma has been developed. The biosensor was fabricated from a d-amino acid oxidase immobilized eggshell membrane and an oxygen electrode. The effects of enzyme loading, glutaraldehyde concentration, pH, ionic strength, and temperature on the response of the biosensor have been investigated in detail. The sensing method is based on monitoring the decrease in the dissolved oxygen level of the sample solution. The linear working range was 0.05-1.5 mM and the limit of detection was 30 μM. The proposed biosensor shows fast response time (<1 min), good repeatability (n = 6, relative standard deviation = 2.38%), and long-term stability (85% of its initial sensitivity after 2 months of storage).

KW - d-Amino acid oxidase

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KW - Homocysteine biosensor

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A homocysteine biosensor with eggshell membrane as an enzyme immobilization platform

Abstract

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