A highly sensitive method for detection of protein based on inhibition of Ru(bpy)32+/TPrA electrochemiluminescent system

Bin Qiu, Xiaofen Jiang, Longhua Guo, Zhenyu Lin*, Zongwei CAI, Guonan Chen

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

The detection of proteins is very important in the study of biochemistry procedure, and electrochemiluminescence method is a very practical tool for the study of protein folding, structure and quantification. In this work, bovine serum albumin (BSA) and casein were found to be able to significantly quench the electrochemiluminescence (ECL) of Ru(bpy)32+/TPrA system, based on which a highly sensitive approach for the detection of protein was proposed. Under the optimized conditions, the logarithmic plot of the inhibited ECL versus the concentration of BSA and casein were linear over the ranges of 1-30 μg/L and 0.1-1.6 μg/L, respectively. The corresponding limit of detection (LOD) was 0.45 μg/L for BSA and 0.026 μg/L for casein (S/N = 3). UV, ECL and fluorescence methods were used to investigate the mechanism of the inhibited ECL of Ru(bpy)32+/TPrA/BSA system. A mechanism based on the formation of protein-Ru(bpy)32+ super molecule was proposed, which would prevent Ru(bpy)32+ from reaching the working electrode surface so that induced ECL quenching.

Original languageEnglish
Pages (from-to)6962-6965
Number of pages4
JournalElectrochimica Acta
Volume56
Issue number20
DOIs
Publication statusPublished - 1 Aug 2011

Scopus Subject Areas

  • Chemical Engineering(all)
  • Electrochemistry

User-Defined Keywords

  • Inhibited electrochemiluminescence
  • Protein
  • Ru(bpy)
  • TPrA

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