A highly sensitive method for detection of protein based on inhibition of Ru(bpy)32+/TPrA electrochemiluminescent system

Bin Qiu; Xiaofen Jiang; Longhua Guo; Zhenyu Lin; Zongwei CAI; Guonan Chen

doi:10.1016/j.electacta.2011.06.016

A highly sensitive method for detection of protein based on inhibition of Ru(bpy)₃²⁺/TPrA electrochemiluminescent system

Bin Qiu, Xiaofen Jiang, Longhua Guo, Zhenyu Lin^*, Zongwei CAI, Guonan Chen

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

13 Citations (Scopus)

Abstract

The detection of proteins is very important in the study of biochemistry procedure, and electrochemiluminescence method is a very practical tool for the study of protein folding, structure and quantification. In this work, bovine serum albumin (BSA) and casein were found to be able to significantly quench the electrochemiluminescence (ECL) of Ru(bpy)₃²⁺/TPrA system, based on which a highly sensitive approach for the detection of protein was proposed. Under the optimized conditions, the logarithmic plot of the inhibited ECL versus the concentration of BSA and casein were linear over the ranges of 1-30 μg/L and 0.1-1.6 μg/L, respectively. The corresponding limit of detection (LOD) was 0.45 μg/L for BSA and 0.026 μg/L for casein (S/N = 3). UV, ECL and fluorescence methods were used to investigate the mechanism of the inhibited ECL of Ru(bpy)₃²⁺/TPrA/BSA system. A mechanism based on the formation of protein-Ru(bpy)₃²⁺ super molecule was proposed, which would prevent Ru(bpy)₃²⁺ from reaching the working electrode surface so that induced ECL quenching.

Original language	English
Pages (from-to)	6962-6965
Number of pages	4
Journal	Electrochimica Acta
Volume	56
Issue number	20
DOIs	https://doi.org/10.1016/j.electacta.2011.06.016
Publication status	Published - 1 Aug 2011

Scopus Subject Areas

Chemical Engineering(all)
Electrochemistry

User-Defined Keywords

Inhibited electrochemiluminescence
Protein
Ru(bpy)
TPrA

Access to Document

10.1016/j.electacta.2011.06.016

Cite this

@article{01ed6efefeac4705a9c44e96b76b2e98,

title = "A highly sensitive method for detection of protein based on inhibition of Ru(bpy)32+/TPrA electrochemiluminescent system",

abstract = "The detection of proteins is very important in the study of biochemistry procedure, and electrochemiluminescence method is a very practical tool for the study of protein folding, structure and quantification. In this work, bovine serum albumin (BSA) and casein were found to be able to significantly quench the electrochemiluminescence (ECL) of Ru(bpy)32+/TPrA system, based on which a highly sensitive approach for the detection of protein was proposed. Under the optimized conditions, the logarithmic plot of the inhibited ECL versus the concentration of BSA and casein were linear over the ranges of 1-30 μg/L and 0.1-1.6 μg/L, respectively. The corresponding limit of detection (LOD) was 0.45 μg/L for BSA and 0.026 μg/L for casein (S/N = 3). UV, ECL and fluorescence methods were used to investigate the mechanism of the inhibited ECL of Ru(bpy)32+/TPrA/BSA system. A mechanism based on the formation of protein-Ru(bpy)32+ super molecule was proposed, which would prevent Ru(bpy)32+ from reaching the working electrode surface so that induced ECL quenching.",

keywords = "Inhibited electrochemiluminescence, Protein, Ru(bpy), TPrA",

author = "Bin Qiu and Xiaofen Jiang and Longhua Guo and Zhenyu Lin and Zongwei CAI and Guonan Chen",

note = "Funding Information: This project was supported by the NSFC ( 41076059 , 20905013 , 20928005 ), the Key Natural Sciences Foundation of Fujian Province, China ( D0520001 ).",

year = "2011",

month = aug,

day = "1",

doi = "10.1016/j.electacta.2011.06.016",

language = "English",

volume = "56",

pages = "6962--6965",

journal = "Electrochimica Acta",

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TY - JOUR

T1 - A highly sensitive method for detection of protein based on inhibition of Ru(bpy)32+/TPrA electrochemiluminescent system

AU - Qiu, Bin

AU - Jiang, Xiaofen

AU - Guo, Longhua

AU - Lin, Zhenyu

AU - CAI, Zongwei

AU - Chen, Guonan

N1 - Funding Information: This project was supported by the NSFC ( 41076059 , 20905013 , 20928005 ), the Key Natural Sciences Foundation of Fujian Province, China ( D0520001 ).

PY - 2011/8/1

Y1 - 2011/8/1

N2 - The detection of proteins is very important in the study of biochemistry procedure, and electrochemiluminescence method is a very practical tool for the study of protein folding, structure and quantification. In this work, bovine serum albumin (BSA) and casein were found to be able to significantly quench the electrochemiluminescence (ECL) of Ru(bpy)32+/TPrA system, based on which a highly sensitive approach for the detection of protein was proposed. Under the optimized conditions, the logarithmic plot of the inhibited ECL versus the concentration of BSA and casein were linear over the ranges of 1-30 μg/L and 0.1-1.6 μg/L, respectively. The corresponding limit of detection (LOD) was 0.45 μg/L for BSA and 0.026 μg/L for casein (S/N = 3). UV, ECL and fluorescence methods were used to investigate the mechanism of the inhibited ECL of Ru(bpy)32+/TPrA/BSA system. A mechanism based on the formation of protein-Ru(bpy)32+ super molecule was proposed, which would prevent Ru(bpy)32+ from reaching the working electrode surface so that induced ECL quenching.

AB - The detection of proteins is very important in the study of biochemistry procedure, and electrochemiluminescence method is a very practical tool for the study of protein folding, structure and quantification. In this work, bovine serum albumin (BSA) and casein were found to be able to significantly quench the electrochemiluminescence (ECL) of Ru(bpy)32+/TPrA system, based on which a highly sensitive approach for the detection of protein was proposed. Under the optimized conditions, the logarithmic plot of the inhibited ECL versus the concentration of BSA and casein were linear over the ranges of 1-30 μg/L and 0.1-1.6 μg/L, respectively. The corresponding limit of detection (LOD) was 0.45 μg/L for BSA and 0.026 μg/L for casein (S/N = 3). UV, ECL and fluorescence methods were used to investigate the mechanism of the inhibited ECL of Ru(bpy)32+/TPrA/BSA system. A mechanism based on the formation of protein-Ru(bpy)32+ super molecule was proposed, which would prevent Ru(bpy)32+ from reaching the working electrode surface so that induced ECL quenching.

KW - Inhibited electrochemiluminescence

KW - Protein

KW - Ru(bpy)

KW - TPrA

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DO - 10.1016/j.electacta.2011.06.016

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JO - Electrochimica Acta

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