A highly sensitive G-quadruplex-based luminescent switch-on probe for the detection of polymerase 3'-5' proofreading activity

Ka Ho Leung; Hong Zhang He; Hai Jing Zhong; Lihua Lu; Daniel Shiu Hin Chan; Dik Lung Ma; Chung Hang Leung

doi:10.1016/j.ymeth.2013.05.017

A highly sensitive G-quadruplex-based luminescent switch-on probe for the detection of polymerase 3'-5' proofreading activity

Ka Ho Leung
, Hong Zhang He
, Hai Jing Zhong
, Lihua Lu
, Daniel Shiu Hin Chan
, Dik Lung Ma^*
, Chung Hang Leung

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

15 Citations (Scopus)

Abstract

We report herein a luminescent switch-on label-free G-quadruplex-based assay for the rapid and sensitive detection of polymerase proofreading activity using a novel iridium(III) complex as a G-quadruplex-selective probe. The interaction of the iridium(III) complex with the G-quadruplex motif facilitates the highly sensitive switch-on detection of polymerase proofreading activity. Using T4 DNA polymerase (T4 pol) as a model enzyme, the assay achieved high sensitivity and selectivity for T4 pol over other tested enzymes.

Original language	English
Pages (from-to)	224-228
Number of pages	5
Journal	Methods
Volume	64
Issue number	3
DOIs	https://doi.org/10.1016/j.ymeth.2013.05.017
Publication status	Published - 15 Dec 2013

User-Defined Keywords

G-quadruplex
Iridium(III) complex
Luminescent
Polymerase
Proofreading

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10.1016/j.ymeth.2013.05.017

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title = "A highly sensitive G-quadruplex-based luminescent switch-on probe for the detection of polymerase 3'-5' proofreading activity",

abstract = "We report herein a luminescent switch-on label-free G-quadruplex-based assay for the rapid and sensitive detection of polymerase proofreading activity using a novel iridium(III) complex as a G-quadruplex-selective probe. The interaction of the iridium(III) complex with the G-quadruplex motif facilitates the highly sensitive switch-on detection of polymerase proofreading activity. Using T4 DNA polymerase (T4 pol) as a model enzyme, the assay achieved high sensitivity and selectivity for T4 pol over other tested enzymes.",

keywords = "G-quadruplex, Iridium(III) complex, Luminescent, Polymerase, Proofreading",

author = "Leung, \{Ka Ho\} and He, \{Hong Zhang\} and Zhong, \{Hai Jing\} and Lihua Lu and Chan, \{Daniel Shiu Hin\} and Ma, \{Dik Lung\} and Leung, \{Chung Hang\}",

note = "Funding Information: This work is supported by Hong Kong Baptist University ( FRG2/11-12/009 ), Centre for Cancer and Inflammation Research, School of Chinese Medicine ( CCIR-SCM, HKBU ), the Health and Medical Research Fund ( HMRF/11101212 ), the Research Grants Council ( HKBU/201811 and HKBU/204612 ), the French National Research Agency/Research Grants Council Joint Research Scheme ( A-HKBU201/12 ), the Science and Technology Development Fund, Macao SAR ( 001/2012/A ) and the University of Macau ( MYRG091(Y2-L2)-ICMS12-LCH and MYRG121(Y2-L2)-ICMS12-LCH ). ",

year = "2013",

month = dec,

day = "15",

doi = "10.1016/j.ymeth.2013.05.017",

language = "English",

volume = "64",

pages = "224--228",

journal = "Methods",

issn = "1046-2023",

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TY - JOUR

T1 - A highly sensitive G-quadruplex-based luminescent switch-on probe for the detection of polymerase 3'-5' proofreading activity

AU - Leung, Ka Ho

AU - He, Hong Zhang

AU - Zhong, Hai Jing

AU - Lu, Lihua

AU - Chan, Daniel Shiu Hin

AU - Ma, Dik Lung

AU - Leung, Chung Hang

N1 - Funding Information: This work is supported by Hong Kong Baptist University ( FRG2/11-12/009 ), Centre for Cancer and Inflammation Research, School of Chinese Medicine ( CCIR-SCM, HKBU ), the Health and Medical Research Fund ( HMRF/11101212 ), the Research Grants Council ( HKBU/201811 and HKBU/204612 ), the French National Research Agency/Research Grants Council Joint Research Scheme ( A-HKBU201/12 ), the Science and Technology Development Fund, Macao SAR ( 001/2012/A ) and the University of Macau ( MYRG091(Y2-L2)-ICMS12-LCH and MYRG121(Y2-L2)-ICMS12-LCH ).

PY - 2013/12/15

Y1 - 2013/12/15

N2 - We report herein a luminescent switch-on label-free G-quadruplex-based assay for the rapid and sensitive detection of polymerase proofreading activity using a novel iridium(III) complex as a G-quadruplex-selective probe. The interaction of the iridium(III) complex with the G-quadruplex motif facilitates the highly sensitive switch-on detection of polymerase proofreading activity. Using T4 DNA polymerase (T4 pol) as a model enzyme, the assay achieved high sensitivity and selectivity for T4 pol over other tested enzymes.

AB - We report herein a luminescent switch-on label-free G-quadruplex-based assay for the rapid and sensitive detection of polymerase proofreading activity using a novel iridium(III) complex as a G-quadruplex-selective probe. The interaction of the iridium(III) complex with the G-quadruplex motif facilitates the highly sensitive switch-on detection of polymerase proofreading activity. Using T4 DNA polymerase (T4 pol) as a model enzyme, the assay achieved high sensitivity and selectivity for T4 pol over other tested enzymes.

KW - G-quadruplex

KW - Iridium(III) complex

KW - Luminescent

KW - Polymerase

KW - Proofreading

UR - https://www.scopus.com/pages/publications/84888247355

U2 - 10.1016/j.ymeth.2013.05.017

DO - 10.1016/j.ymeth.2013.05.017

M3 - Journal article

C2 - 23748144

AN - SCOPUS:84888247355

SN - 1046-2023

VL - 64

SP - 224

EP - 228

JO - Methods

JF - Methods

IS - 3

ER -

A highly sensitive G-quadruplex-based luminescent switch-on probe for the detection of polymerase 3'-5' proofreading activity

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