@article{7efbefacef4441dbac8240a605914bd6,
title = "A highly sensitive G-quadruplex-based luminescent switch-on probe for the detection of polymerase 3'-5' proofreading activity",
abstract = "We report herein a luminescent switch-on label-free G-quadruplex-based assay for the rapid and sensitive detection of polymerase proofreading activity using a novel iridium(III) complex as a G-quadruplex-selective probe. The interaction of the iridium(III) complex with the G-quadruplex motif facilitates the highly sensitive switch-on detection of polymerase proofreading activity. Using T4 DNA polymerase (T4 pol) as a model enzyme, the assay achieved high sensitivity and selectivity for T4 pol over other tested enzymes.",
keywords = "G-quadruplex, Iridium(III) complex, Luminescent, Polymerase, Proofreading",
author = "Leung, {Ka Ho} and He, {Hong Zhang} and Zhong, {Hai Jing} and Lihua Lu and Chan, {Daniel Shiu Hin} and Ma, {Dik Lung} and Leung, {Chung Hang}",
note = "Funding Information: This work is supported by Hong Kong Baptist University ( FRG2/11-12/009 ), Centre for Cancer and Inflammation Research, School of Chinese Medicine ( CCIR-SCM, HKBU ), the Health and Medical Research Fund ( HMRF/11101212 ), the Research Grants Council ( HKBU/201811 and HKBU/204612 ), the French National Research Agency/Research Grants Council Joint Research Scheme ( A-HKBU201/12 ), the Science and Technology Development Fund, Macao SAR ( 001/2012/A ) and the University of Macau ( MYRG091(Y2-L2)-ICMS12-LCH and MYRG121(Y2-L2)-ICMS12-LCH ). ",
year = "2013",
month = dec,
day = "15",
doi = "10.1016/j.ymeth.2013.05.017",
language = "English",
volume = "64",
pages = "224--228",
journal = "Methods",
issn = "1046-2023",
publisher = "Academic Press Inc.",
number = "3",
}