A highly selective luminescent switch-on probe for histidine/histidine-rich proteins and its application in protein staining

Dik Lung Ma; Wing Leung Wong; Wai Hong Chung; Fung Yi Chan; Pui Kin So; Tat Shing Lai; Zhong Yuan Zhou; Yun Chung Leung; Kwok Yin Wong

doi:10.1002/anie.200705319

A highly selective luminescent switch-on probe for histidine/histidine-rich proteins and its application in protein staining

Dik Lung Ma^*
, Wing Leung Wong
, Wai Hong Chung
, Fung Yi Chan
, Pui Kin So
, Tat Shing Lai
, Zhong Yuan Zhou
, Yun Chung Leung
, Kwok Yin Wong

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

229 Citations (Scopus)

Abstract

No destaining required: The luminescence properties of an iridium(III)-2-phenylpyridine complex can be utilized for the detection of proteins in sodium dodecyl sulfate-polyacrylamide gels. Emissive gel images obtained upon staining with the complex are visible under UV light. The high detection sensitivity for histidine-rich proteins (right vial, compared to a non-histidine-tagged protein) suggests potential applications in the signaling of biomolecules. (Figure Presented).

Original language	English
Pages (from-to)	3735-3739
Number of pages	5
Journal	Angewandte Chemie. International Edition
Volume	47
Issue number	20
DOIs	https://doi.org/10.1002/anie.200705319
Publication status	Published - May 2008

User-Defined Keywords

Electrophoresis
Histidine
Iridium
Luminescent probes
Protein staining

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10.1002/anie.200705319

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title = "A highly selective luminescent switch-on probe for histidine/histidine-rich proteins and its application in protein staining",

abstract = "No destaining required: The luminescence properties of an iridium(III)-2-phenylpyridine complex can be utilized for the detection of proteins in sodium dodecyl sulfate-polyacrylamide gels. Emissive gel images obtained upon staining with the complex are visible under UV light. The high detection sensitivity for histidine-rich proteins (right vial, compared to a non-histidine-tagged protein) suggests potential applications in the signaling of biomolecules. (Figure Presented).",

keywords = "Electrophoresis, Histidine, Iridium, Luminescent probes, Protein staining",

author = "Ma, \{Dik Lung\} and Wong, \{Wing Leung\} and Chung, \{Wai Hong\} and Chan, \{Fung Yi\} and So, \{Pui Kin\} and Lai, \{Tat Shing\} and Zhou, \{Zhong Yuan\} and Leung, \{Yun Chung\} and Wong, \{Kwok Yin\}",

year = "2008",

month = may,

doi = "10.1002/anie.200705319",

language = "English",

volume = "47",

pages = "3735--3739",

journal = "Angewandte Chemie. International Edition",

issn = "1433-7851",

publisher = "John Wiley \& Sons Ltd",

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TY - JOUR

T1 - A highly selective luminescent switch-on probe for histidine/histidine-rich proteins and its application in protein staining

AU - Ma, Dik Lung

AU - Wong, Wing Leung

AU - Chung, Wai Hong

AU - Chan, Fung Yi

AU - So, Pui Kin

AU - Lai, Tat Shing

AU - Zhou, Zhong Yuan

AU - Leung, Yun Chung

AU - Wong, Kwok Yin

PY - 2008/5

Y1 - 2008/5

N2 - No destaining required: The luminescence properties of an iridium(III)-2-phenylpyridine complex can be utilized for the detection of proteins in sodium dodecyl sulfate-polyacrylamide gels. Emissive gel images obtained upon staining with the complex are visible under UV light. The high detection sensitivity for histidine-rich proteins (right vial, compared to a non-histidine-tagged protein) suggests potential applications in the signaling of biomolecules. (Figure Presented).

AB - No destaining required: The luminescence properties of an iridium(III)-2-phenylpyridine complex can be utilized for the detection of proteins in sodium dodecyl sulfate-polyacrylamide gels. Emissive gel images obtained upon staining with the complex are visible under UV light. The high detection sensitivity for histidine-rich proteins (right vial, compared to a non-histidine-tagged protein) suggests potential applications in the signaling of biomolecules. (Figure Presented).

KW - Electrophoresis

KW - Histidine

KW - Iridium

KW - Luminescent probes

KW - Protein staining

UR - https://www.scopus.com/pages/publications/45549095723

U2 - 10.1002/anie.200705319

DO - 10.1002/anie.200705319

M3 - Journal article

C2 - 18412213

AN - SCOPUS:45549095723

SN - 1433-7851

VL - 47

SP - 3735

EP - 3739

JO - Angewandte Chemie. International Edition

JF - Angewandte Chemie. International Edition

IS - 20

ER -

A highly selective luminescent switch-on probe for histidine/histidine-rich proteins and its application in protein staining

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