A highly selective luminescent switch-on probe for histidine/histidine-rich proteins and its application in protein staining

Dik Lung Ma*, Wing Leung Wong, Wai Hong Chung, Fung Yi Chan, Pui Kin So, Tat Shing Lai, Zhong Yuan Zhou, Yun Chung Leung, Kwok Yin Wong

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

195 Citations (Scopus)

Abstract

No destaining required: The luminescence properties of an iridium(III)-2-phenylpyridine complex can be utilized for the detection of proteins in sodium dodecyl sulfate-polyacrylamide gels. Emissive gel images obtained upon staining with the complex are visible under UV light. The high detection sensitivity for histidine-rich proteins (right vial, compared to a non-histidine-tagged protein) suggests potential applications in the signaling of biomolecules. (Figure Presented).

Original languageEnglish
Pages (from-to)3735-3739
Number of pages5
JournalAngewandte Chemie - International Edition
Volume47
Issue number20
DOIs
Publication statusPublished - May 2008
Externally publishedYes

Scopus Subject Areas

  • Catalysis
  • Chemistry(all)

User-Defined Keywords

  • Electrophoresis
  • Histidine
  • Iridium
  • Luminescent probes
  • Protein staining

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