A highly selective luminescent switch-on probe for histidine/histidine-rich proteins and its application in protein staining

Dik Lung Ma; Wing Leung Wong; Wai Hong Chung; Fung Yi Chan; Pui Kin So; Tat Shing Lai; Zhong Yuan Zhou; Yun Chung Leung; Kwok Yin Wong

doi:10.1002/anie.200705319

A highly selective luminescent switch-on probe for histidine/histidine-rich proteins and its application in protein staining

Dik Lung Ma^*, Wing Leung Wong, Wai Hong Chung, Fung Yi Chan, Pui Kin So, Tat Shing Lai, Zhong Yuan Zhou, Yun Chung Leung, Kwok Yin Wong

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

224 Citations (Scopus)

Abstract

No destaining required: The luminescence properties of an iridium(III)-2-phenylpyridine complex can be utilized for the detection of proteins in sodium dodecyl sulfate-polyacrylamide gels. Emissive gel images obtained upon staining with the complex are visible under UV light. The high detection sensitivity for histidine-rich proteins (right vial, compared to a non-histidine-tagged protein) suggests potential applications in the signaling of biomolecules. (Figure Presented).

Original language	English
Pages (from-to)	3735-3739
Number of pages	5
Journal	Angewandte Chemie. International Edition
Volume	47
Issue number	20
DOIs	https://doi.org/10.1002/anie.200705319
Publication status	Published - May 2008

Scopus Subject Areas

Catalysis
General Chemistry

User-Defined Keywords

Electrophoresis
Histidine
Iridium
Luminescent probes
Protein staining

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10.1002/anie.200705319

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title = "A highly selective luminescent switch-on probe for histidine/histidine-rich proteins and its application in protein staining",

abstract = "No destaining required: The luminescence properties of an iridium(III)-2-phenylpyridine complex can be utilized for the detection of proteins in sodium dodecyl sulfate-polyacrylamide gels. Emissive gel images obtained upon staining with the complex are visible under UV light. The high detection sensitivity for histidine-rich proteins (right vial, compared to a non-histidine-tagged protein) suggests potential applications in the signaling of biomolecules. (Figure Presented).",

keywords = "Electrophoresis, Histidine, Iridium, Luminescent probes, Protein staining",

author = "Ma, {Dik Lung} and Wong, {Wing Leung} and Chung, {Wai Hong} and Chan, {Fung Yi} and So, {Pui Kin} and Lai, {Tat Shing} and Zhou, {Zhong Yuan} and Leung, {Yun Chung} and Wong, {Kwok Yin}",

year = "2008",

month = may,

doi = "10.1002/anie.200705319",

language = "English",

volume = "47",

pages = "3735--3739",

journal = "Angewandte Chemie. International Edition",

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T1 - A highly selective luminescent switch-on probe for histidine/histidine-rich proteins and its application in protein staining

AU - Ma, Dik Lung

AU - Wong, Wing Leung

AU - Chung, Wai Hong

AU - Chan, Fung Yi

AU - So, Pui Kin

AU - Lai, Tat Shing

AU - Zhou, Zhong Yuan

AU - Leung, Yun Chung

AU - Wong, Kwok Yin

PY - 2008/5

Y1 - 2008/5

N2 - No destaining required: The luminescence properties of an iridium(III)-2-phenylpyridine complex can be utilized for the detection of proteins in sodium dodecyl sulfate-polyacrylamide gels. Emissive gel images obtained upon staining with the complex are visible under UV light. The high detection sensitivity for histidine-rich proteins (right vial, compared to a non-histidine-tagged protein) suggests potential applications in the signaling of biomolecules. (Figure Presented).

AB - No destaining required: The luminescence properties of an iridium(III)-2-phenylpyridine complex can be utilized for the detection of proteins in sodium dodecyl sulfate-polyacrylamide gels. Emissive gel images obtained upon staining with the complex are visible under UV light. The high detection sensitivity for histidine-rich proteins (right vial, compared to a non-histidine-tagged protein) suggests potential applications in the signaling of biomolecules. (Figure Presented).

KW - Electrophoresis

KW - Histidine

KW - Iridium

KW - Luminescent probes

KW - Protein staining

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U2 - 10.1002/anie.200705319

DO - 10.1002/anie.200705319

M3 - Journal article

C2 - 18412213

AN - SCOPUS:45549095723

SN - 1433-7851

VL - 47

SP - 3735

EP - 3739

JO - Angewandte Chemie. International Edition

JF - Angewandte Chemie. International Edition

IS - 20

ER -

A highly selective luminescent switch-on probe for histidine/histidine-rich proteins and its application in protein staining

Abstract

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