Abstract
1. Arrayed primer extension (APEX) has been successfully developed as a high-throughput method for single nucleotide polymorphisms (SNPs) present in the hepatitis B virus (HBV) genome.
2. Co-existence of wild-type and mutant genotypes in one SNP was successfully identified by APEX, which was not identified by conventional DNA sequencing.
3. Thirty-three patients were involved in this study; APEX results showed A1762T and G1764A double mutations and G1896A mutation are the most prevalent SNPs present in HBV genomes.
4. APEX can be developed as a SNP screening tool to monitor other infectious agents.
2. Co-existence of wild-type and mutant genotypes in one SNP was successfully identified by APEX, which was not identified by conventional DNA sequencing.
3. Thirty-three patients were involved in this study; APEX results showed A1762T and G1764A double mutations and G1896A mutation are the most prevalent SNPs present in HBV genomes.
4. APEX can be developed as a SNP screening tool to monitor other infectious agents.
| Original language | English |
|---|---|
| Pages (from-to) | S4-S7 |
| Number of pages | 4 |
| Journal | Hong Kong Medical Journal |
| Volume | 14 |
| Issue number | 5, Suppl 5 |
| Publication status | Published - Oct 2008 |
