A high-throughput detection method for hepatitis B virus mutations in the Chinese population

C. C. Lau; W. Y. Ha; Patrick Y K YUE; S. H. Chui; A. K. K. Chui; Ricky N S WONG

A high-throughput detection method for hepatitis B virus mutations in the Chinese population

C. C. Lau, W. Y. Ha, Patrick Y K YUE, S. H. Chui, A. K. K. Chui, Ricky N S WONG^*

^*Corresponding author for this work

Department of Biology

Research output: Contribution to journal › Journal article › peer-review

Abstract

1. Arrayed primer extension (APEX) has been successfully developed as a high-throughput method for single nucleotide polymorphisms (SNPs) present in the hepatitis B virus (HBV) genome.
2. Co-existence of wild-type and mutant genotypes in one SNP was successfully identified by APEX, which was not identified by conventional DNA sequencing.
3. Thirty-three patients were involved in this study; APEX results showed A1762T and G1764A double mutations and G1896A mutation are the most prevalent SNPs present in HBV genomes.
4. APEX can be developed as a SNP screening tool to monitor other infectious agents.

Original language	English
Pages (from-to)	S4-S7
Number of pages	4
Journal	Hong Kong Medical Journal
Volume	14
Issue number	5, Suppl 5
Publication status	Published - Oct 2008

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@article{134e634190ad496eb0b011874d06755e,

title = "A high-throughput detection method for hepatitis B virus mutations in the Chinese population",

abstract = "1. Arrayed primer extension (APEX) has been successfully developed as a high-throughput method for single nucleotide polymorphisms (SNPs) present in the hepatitis B virus (HBV) genome.2. Co-existence of wild-type and mutant genotypes in one SNP was successfully identified by APEX, which was not identified by conventional DNA sequencing.3. Thirty-three patients were involved in this study; APEX results showed A1762T and G1764A double mutations and G1896A mutation are the most prevalent SNPs present in HBV genomes.4. APEX can be developed as a SNP screening tool to monitor other infectious agents.",

author = "Lau, {C. C.} and Ha, {W. Y.} and YUE, {Patrick Y K} and Chui, {S. H.} and Chui, {A. K. K.} and WONG, {Ricky N S}",

note = "This study was supported by the Research Fund for the Control of Infectious Diseases, Food and Health Bureau, Hong Kong SAR Government (RFCID: 01030072).",

year = "2008",

month = oct,

language = "English",

volume = "14",

pages = "S4--S7",

journal = "Hong Kong Medical Journal",

issn = "1024-2708",

publisher = "Hong Kong Academy of Medicine Press",

number = "5, Suppl 5",

}

TY - JOUR

T1 - A high-throughput detection method for hepatitis B virus mutations in the Chinese population

AU - Lau, C. C.

AU - Ha, W. Y.

AU - YUE, Patrick Y K

AU - Chui, S. H.

AU - Chui, A. K. K.

AU - WONG, Ricky N S

N1 - This study was supported by the Research Fund for the Control of Infectious Diseases, Food and Health Bureau, Hong Kong SAR Government (RFCID: 01030072).

PY - 2008/10

Y1 - 2008/10

N2 - 1. Arrayed primer extension (APEX) has been successfully developed as a high-throughput method for single nucleotide polymorphisms (SNPs) present in the hepatitis B virus (HBV) genome.2. Co-existence of wild-type and mutant genotypes in one SNP was successfully identified by APEX, which was not identified by conventional DNA sequencing.3. Thirty-three patients were involved in this study; APEX results showed A1762T and G1764A double mutations and G1896A mutation are the most prevalent SNPs present in HBV genomes.4. APEX can be developed as a SNP screening tool to monitor other infectious agents.

AB - 1. Arrayed primer extension (APEX) has been successfully developed as a high-throughput method for single nucleotide polymorphisms (SNPs) present in the hepatitis B virus (HBV) genome.2. Co-existence of wild-type and mutant genotypes in one SNP was successfully identified by APEX, which was not identified by conventional DNA sequencing.3. Thirty-three patients were involved in this study; APEX results showed A1762T and G1764A double mutations and G1896A mutation are the most prevalent SNPs present in HBV genomes.4. APEX can be developed as a SNP screening tool to monitor other infectious agents.

UR - https://www.hkmj.org/abstracts/v14n5%20Suppl/4.htm

UR - http://www.scopus.com/inward/record.url?scp=60549093931&partnerID=8YFLogxK

M3 - Journal article

C2 - 18941265

AN - SCOPUS:60549093931

SN - 1024-2708

VL - 14

SP - S4-S7

JO - Hong Kong Medical Journal

JF - Hong Kong Medical Journal

IS - 5, Suppl 5

ER -

A high-throughput detection method for hepatitis B virus mutations in the Chinese population

Abstract

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