A herbal formula consisting of Rosae Multiflorae Fructus and Lonicerae Japonicae Flos inhibits inflammatory mediators in LPS-stimulated RAW 264.7 macrophages

Brian Chi Yan Cheng, Xiao Qing Ma, Hiu Yee KWAN, Anfernee K W TSE, Hui Hui Cao, Tao Su, Xin Shu, Zheng Zhi Wu, Zhiling YU*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

44 Citations (Scopus)

Abstract

Ethnopharmacological relevance A herbal formula (RL) consisting of Rosae Multiflorae Fructus (Yingshi) and Lonicerae Japonicae Flos (Jinyinhua) has been traditionally used to treat inflammatory disorders. This study aims to investigate the anti-inflammatory mode and mechanism of action of the ethanol extract of RL so as to provide a pharmacological basis for the use of RL in treating inflammatory diseases. Materials and method RL consisting of Yingshi and Jinyinhua (in 5:3 ratio) was extracted using absolute ethanol. We investigated its effects on nitric oxide (NO), interleukin-6 (IL-6), tumour necrosis factor (TNF)-α, inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), mitogen-activated protein kinases (MAPKs) and nuclear factor κB (NFκB) in mouse RAW 264.7 macrophages activated with lipopolysaccharide (LPS). Results RL could decrease the secretion of NO, IL-6 and TNF-α into the culture medium and the cellular protein levels of iNOS and COX-2, which were associated with the reduction of the phosphorylation/ activation of JNK and p38, and the inhibition of the transcriptional activity of NF-κB. Conclusions The present study demonstrated an inhibitory effect of RL on the inflammatory mediators regulated by the NF-κB and MAPK signalling pathways in LPS-stimulated RAW 264.7 macrophages, providing a pharmacological basis for RL in the control of inflammatory disorders.

Original languageEnglish
Pages (from-to)922-927
Number of pages6
JournalJournal of Ethnopharmacology
Volume153
Issue number3
DOIs
Publication statusPublished - 14 May 2014

Scopus Subject Areas

  • Pharmacology
  • Drug Discovery

User-Defined Keywords

  • Anti-inflammation
  • Lonicera japonica
  • MAPK
  • NF-κB
  • Rosa multiflora

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