A G-triplex luminescent switch-on probe for the detection of mung bean nuclease activity

Edmond Dik Lung Ma; Lihua Lu; Sheng Lin; Bingyong He; Chung Hang Leung

doi:10.1039/c4tb01569j

A G-triplex luminescent switch-on probe for the detection of mung bean nuclease activity

Edmond Dik Lung Ma^*
, Lihua Lu
, Sheng Lin
, Bingyong He
, Chung Hang Leung

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

17 Citations (Scopus)

Abstract

A cyclometallated Ir(III) complex was synthesised and evaluated for the ability to act as a luminescent G-triplex probe. The iridium(III) complex 1 [Ir(phq)₂(2,9-dmphen)]PF₆ (where phq = 2-phenylquinoline; 2,9-dmphen = 2,9-dimethyl-1,10-phenanthroline) exhibited high luminescence for G-triplex DNA over dsDNA and ssDNA, and was employed to construct a label-free G-triplex-based assay for mung bean nuclease activity in aqueous solution. This method was highly sensitive and selective for MB nuclease activity over other DNA-modifying enzymes.

Original language	English
Pages (from-to)	348-352
Number of pages	5
Journal	Journal of Materials Chemistry B
Volume	3
Issue number	3
Early online date	27 Nov 2014
DOIs	https://doi.org/10.1039/c4tb01569j
Publication status	Published - 21 Jan 2015

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title = "A G-triplex luminescent switch-on probe for the detection of mung bean nuclease activity",

abstract = "A cyclometallated Ir(III) complex was synthesised and evaluated for the ability to act as a luminescent G-triplex probe. The iridium(III) complex 1 [Ir(phq)2(2,9-dmphen)]PF6 (where phq = 2-phenylquinoline; 2,9-dmphen = 2,9-dimethyl-1,10-phenanthroline) exhibited high luminescence for G-triplex DNA over dsDNA and ssDNA, and was employed to construct a label-free G-triplex-based assay for mung bean nuclease activity in aqueous solution. This method was highly sensitive and selective for MB nuclease activity over other DNA-modifying enzymes.",

author = "Ma, \{Edmond Dik Lung\} and Lihua Lu and Sheng Lin and Bingyong He and Leung, \{Chung Hang\}",

note = "Funding information: This work is supported by Hong Kong Baptist University (FRG2/13-14/008), the Centre for Cancer and Inflammation Research, School of Chinese Medicine (CCIR-SCM, HKBU), the Health and Medical Research Fund (HMRF/13121482), the Research Grants Council (HKBU/201811, HKBU/204612 and HKBU/201913), the French National Research Agency/Research Grants Council Joint Research Scheme (A-HKBU201/12), the State Key Laboratory of Synthetic Chemistry, the Science and Technology Development Fund, Macao SAR (103/2012/A3) and the University of Macau (MYRG091(Y3-L2)-ICMS12-LCH, MYRG121(Y3-L2)-ICMS12-LCH and MRG023/LCH/2013/ICMS). Publisher ocpyright: {\textcopyright} The Royal Society of Chemistry 2015",

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doi = "10.1039/c4tb01569j",

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T1 - A G-triplex luminescent switch-on probe for the detection of mung bean nuclease activity

AU - Ma, Edmond Dik Lung

AU - Lu, Lihua

AU - Lin, Sheng

AU - He, Bingyong

AU - Leung, Chung Hang

N1 - Funding information: This work is supported by Hong Kong Baptist University (FRG2/13-14/008), the Centre for Cancer and Inflammation Research, School of Chinese Medicine (CCIR-SCM, HKBU), the Health and Medical Research Fund (HMRF/13121482), the Research Grants Council (HKBU/201811, HKBU/204612 and HKBU/201913), the French National Research Agency/Research Grants Council Joint Research Scheme (A-HKBU201/12), the State Key Laboratory of Synthetic Chemistry, the Science and Technology Development Fund, Macao SAR (103/2012/A3) and the University of Macau (MYRG091(Y3-L2)-ICMS12-LCH, MYRG121(Y3-L2)-ICMS12-LCH and MRG023/LCH/2013/ICMS). Publisher ocpyright: © The Royal Society of Chemistry 2015

PY - 2015/1/21

Y1 - 2015/1/21

N2 - A cyclometallated Ir(III) complex was synthesised and evaluated for the ability to act as a luminescent G-triplex probe. The iridium(III) complex 1 [Ir(phq)2(2,9-dmphen)]PF6 (where phq = 2-phenylquinoline; 2,9-dmphen = 2,9-dimethyl-1,10-phenanthroline) exhibited high luminescence for G-triplex DNA over dsDNA and ssDNA, and was employed to construct a label-free G-triplex-based assay for mung bean nuclease activity in aqueous solution. This method was highly sensitive and selective for MB nuclease activity over other DNA-modifying enzymes.

AB - A cyclometallated Ir(III) complex was synthesised and evaluated for the ability to act as a luminescent G-triplex probe. The iridium(III) complex 1 [Ir(phq)2(2,9-dmphen)]PF6 (where phq = 2-phenylquinoline; 2,9-dmphen = 2,9-dimethyl-1,10-phenanthroline) exhibited high luminescence for G-triplex DNA over dsDNA and ssDNA, and was employed to construct a label-free G-triplex-based assay for mung bean nuclease activity in aqueous solution. This method was highly sensitive and selective for MB nuclease activity over other DNA-modifying enzymes.

UR - https://www.scopus.com/pages/publications/84918799908

U2 - 10.1039/c4tb01569j

DO - 10.1039/c4tb01569j

M3 - Journal article

AN - SCOPUS:84918799908

SN - 2050-750X

VL - 3

SP - 348

EP - 352

JO - Journal of Materials Chemistry B

JF - Journal of Materials Chemistry B

IS - 3

ER -

A G-triplex luminescent switch-on probe for the detection of mung bean nuclease activity

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