A G-triplex luminescent switch-on probe for the detection of mung bean nuclease activity

Edmond Dik Lung Ma; Lihua Lu; Sheng Lin; Bingyong He; Chung Hang Leung

doi:10.1039/c4tb01569j

A G-triplex luminescent switch-on probe for the detection of mung bean nuclease activity

Edmond Dik Lung Ma^*, Lihua Lu, Sheng Lin, Bingyong He, Chung Hang Leung

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

A cyclometallated Ir(III) complex was synthesised and evaluated for the ability to act as a luminescent G-triplex probe. The iridium(III) complex 1 [Ir(phq)₂(2,9-dmphen)]PF₆ (where phq = 2-phenylquinoline; 2,9-dmphen = 2,9-dimethyl-1,10-phenanthroline) exhibited high luminescence for G-triplex DNA over dsDNA and ssDNA, and was employed to construct a label-free G-triplex-based assay for mung bean nuclease activity in aqueous solution. This method was highly sensitive and selective for MB nuclease activity over other DNA-modifying enzymes.

Original language	English
Pages (from-to)	348-352
Number of pages	5
Journal	Journal of Materials Chemistry B
Volume	3
Issue number	3
Early online date	27 Nov 2014
DOIs	https://doi.org/10.1039/c4tb01569j
Publication status	Published - 21 Jan 2015

Scopus Subject Areas

Chemistry(all)
Biomedical Engineering
Materials Science(all)

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title = "A G-triplex luminescent switch-on probe for the detection of mung bean nuclease activity",

abstract = "A cyclometallated Ir(III) complex was synthesised and evaluated for the ability to act as a luminescent G-triplex probe. The iridium(III) complex 1 [Ir(phq)2(2,9-dmphen)]PF6 (where phq = 2-phenylquinoline; 2,9-dmphen = 2,9-dimethyl-1,10-phenanthroline) exhibited high luminescence for G-triplex DNA over dsDNA and ssDNA, and was employed to construct a label-free G-triplex-based assay for mung bean nuclease activity in aqueous solution. This method was highly sensitive and selective for MB nuclease activity over other DNA-modifying enzymes.",

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T1 - A G-triplex luminescent switch-on probe for the detection of mung bean nuclease activity

AU - Ma, Edmond Dik Lung

AU - Lu, Lihua

AU - Lin, Sheng

AU - He, Bingyong

AU - Leung, Chung Hang

PY - 2015/1/21

Y1 - 2015/1/21

N2 - A cyclometallated Ir(III) complex was synthesised and evaluated for the ability to act as a luminescent G-triplex probe. The iridium(III) complex 1 [Ir(phq)2(2,9-dmphen)]PF6 (where phq = 2-phenylquinoline; 2,9-dmphen = 2,9-dimethyl-1,10-phenanthroline) exhibited high luminescence for G-triplex DNA over dsDNA and ssDNA, and was employed to construct a label-free G-triplex-based assay for mung bean nuclease activity in aqueous solution. This method was highly sensitive and selective for MB nuclease activity over other DNA-modifying enzymes.

AB - A cyclometallated Ir(III) complex was synthesised and evaluated for the ability to act as a luminescent G-triplex probe. The iridium(III) complex 1 [Ir(phq)2(2,9-dmphen)]PF6 (where phq = 2-phenylquinoline; 2,9-dmphen = 2,9-dimethyl-1,10-phenanthroline) exhibited high luminescence for G-triplex DNA over dsDNA and ssDNA, and was employed to construct a label-free G-triplex-based assay for mung bean nuclease activity in aqueous solution. This method was highly sensitive and selective for MB nuclease activity over other DNA-modifying enzymes.

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JO - Journal of Materials Chemistry B

JF - Journal of Materials Chemistry B

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ER -

A G-triplex luminescent switch-on probe for the detection of mung bean nuclease activity

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