A G-triplex luminescent switch-on probe for the detection of mung bean nuclease activity

Edmond Dik Lung Ma*, Lihua Lu, Sheng Lin, Bingyong He, Chung Hang Leung

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

15 Citations (Scopus)

Abstract

A cyclometallated Ir(III) complex was synthesised and evaluated for the ability to act as a luminescent G-triplex probe. The iridium(III) complex 1 [Ir(phq)2(2,9-dmphen)]PF6 (where phq = 2-phenylquinoline; 2,9-dmphen = 2,9-dimethyl-1,10-phenanthroline) exhibited high luminescence for G-triplex DNA over dsDNA and ssDNA, and was employed to construct a label-free G-triplex-based assay for mung bean nuclease activity in aqueous solution. This method was highly sensitive and selective for MB nuclease activity over other DNA-modifying enzymes.

Original languageEnglish
Pages (from-to)348-352
Number of pages5
JournalJournal of Materials Chemistry B
Volume3
Issue number3
Early online date27 Nov 2014
DOIs
Publication statusPublished - 21 Jan 2015

Scopus Subject Areas

  • General Chemistry
  • Biomedical Engineering
  • General Materials Science

Fingerprint

Dive into the research topics of 'A G-triplex luminescent switch-on probe for the detection of mung bean nuclease activity'. Together they form a unique fingerprint.

Cite this