@article{477019c924b64a4eb3b548fdfe622402,
title = "A G-quadruplex-selective luminescent probe with an anchor tail for the switch-on detection of thymine DNA glycosylase activity",
abstract = "Thymine DNA glycosylase (TDG) performs essential functions in maintaining genetic integrity and epigenetic regulation, which also plays an essential role in DNA demethylation. In this work, the novel iridium(III) complex 1 with an anchor tail was synthesized and employed to construct a G-quadruplex-based assay for detecting TDG activity in aqueous solution by using the mismatched base excising property of TDG with T4 DNA ligase and phi29 DNA polymerase, in concert with the rolling circle amplification (RCA) strategy. The assay achieved a detection limit of 0.048 U mL−1 (0.012 ng mL−1), and showed high selectivity towards TDG even in the presence of other proteins and enzymes. Additionally, the assay could function in diluted cellular debris.",
keywords = "G-quadruplex, Iridium(III) complex, Luminescence, TDG",
author = "Sheng Lin and Kang, {Tian Shu} and Lihua Lu and Wanhe Wang and Ma, {Edmond Dik Lung} and Leung, {Chung Hang}",
note = "Funding Information: This work is supported by Hong Kong Baptist University ( FRG2/14-15/004 and FRG2/15-16/002 ), the Health and Medical Research Fund ( HMRF/14130522 ), the Research Grants Council ( HKBU/201811 , HKBU/204612 and HKBU/201913 ), the French Agence Nationale de la Recherche/Research Grants Council Joint Research Scheme ( AHKBU201/12 ; Oligoswitch ANR-12-IS07-0001 ), the National Natural Science Foundation of China ( 21575121 ), the Guangdong Province Natural Science Foundation ( 2015A030313816 ), the Hong Kong Baptist University Century Club Sponsorship Scheme 2015 , the Interdisciplinary Research Matching Scheme ( RC-IRMS/14-15/06 ), the Science and Technology Development Fund, Macao SAR ( 098/2014/A2 ), and the University of Macau ( MYRG091(Y3-L2)-ICMS12-LCH , MYRG2015-00137-ICMS-QRCM , and MRG044/LCH/2015/ICMS ). ",
year = "2016",
month = dec,
day = "15",
doi = "10.1016/j.bios.2016.07.082",
language = "English",
volume = "86",
pages = "849--857",
journal = "Biosensors and Bioelectronics",
issn = "0956-5663",
publisher = "Elsevier Ltd.",
}