A feasible and practical 1H NMR analytical method for the quality control and quantification of bioactive principles in Lycii Fructus

Li Ying Hsieh; Hsiu Hui Chan; Ping Chung Kuo; Hsin Yi Hung; Yue Chiun Li; Chao Lin Kuo; Yong Peng; Zhongzhen Zhao; Daih Huang Kuo; I. Wen Sun; Tian Shung Wu

doi:10.1016/j.jfda.2018.01.001

A feasible and practical ¹H NMR analytical method for the quality control and quantification of bioactive principles in Lycii Fructus

Li Ying Hsieh, Hsiu Hui Chan, Ping Chung Kuo, Hsin Yi Hung, Yue Chiun Li, Chao Lin Kuo, Yong Peng, Zhongzhen Zhao, Daih Huang Kuo, I. Wen Sun, Tian Shung Wu^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › peer-review

21 Citations (Scopus)

Abstract

Lycii Fructus, a solanaceous drug, is widely used as functional foods and in Traditional Chinese Medicine. Samples collected from different regions of China have been found to be not identical in chemical compositions which might affect the biological activities. Although many chromatographic and spectrometric methods have been reported to determine the concentration of betaine and other bioactive amino acids, disturbance resulted from other polar substances with low UV-absorbance and expensive mass facilities reduced the applicability of these techniques. In the present study, the strong cation exchange solid phase extraction procedure incorporated with ¹H NMR was successfully developed as a rapid and reliable method that can simultaneously determine betaine, citric acid, threonine, alanine, and proline in various Lycii Fructus. In addition, ERETIC 2 method based on PULCON principle was also applied and compared with conventional method. This feasible and practical method offers a very powerful tool for the quality control of commercial Lycii Fructus from different sources.

Original language	English
Pages (from-to)	1105-1112
Number of pages	8
Journal	Journal of Food and Drug Analysis
Volume	26
Issue number	3
DOIs	https://doi.org/10.1016/j.jfda.2018.01.001
Publication status	Published - Jul 2018

User-Defined Keywords

H NMR
Betaine
ERETIC 2
Lycii Fructus
PULCON

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10.1016/j.jfda.2018.01.001

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title = "A feasible and practical 1H NMR analytical method for the quality control and quantification of bioactive principles in Lycii Fructus",

abstract = "Lycii Fructus, a solanaceous drug, is widely used as functional foods and in Traditional Chinese Medicine. Samples collected from different regions of China have been found to be not identical in chemical compositions which might affect the biological activities. Although many chromatographic and spectrometric methods have been reported to determine the concentration of betaine and other bioactive amino acids, disturbance resulted from other polar substances with low UV-absorbance and expensive mass facilities reduced the applicability of these techniques. In the present study, the strong cation exchange solid phase extraction procedure incorporated with 1H NMR was successfully developed as a rapid and reliable method that can simultaneously determine betaine, citric acid, threonine, alanine, and proline in various Lycii Fructus. In addition, ERETIC 2 method based on PULCON principle was also applied and compared with conventional method. This feasible and practical method offers a very powerful tool for the quality control of commercial Lycii Fructus from different sources.",

keywords = "H NMR, Betaine, ERETIC 2, Lycii Fructus, PULCON",

author = "Hsieh, {Li Ying} and Chan, {Hsiu Hui} and Kuo, {Ping Chung} and Hung, {Hsin Yi} and Li, {Yue Chiun} and Kuo, {Chao Lin} and Yong Peng and Zhongzhen Zhao and Kuo, {Daih Huang} and Sun, {I. Wen} and Wu, {Tian Shung}",

note = "Funding Information: This study was supported by the Ministry of Science and Technology, Taiwan , to T-S. Wu. ",

year = "2018",

month = jul,

doi = "10.1016/j.jfda.2018.01.001",

language = "English",

volume = "26",

pages = "1105--1112",

journal = "Journal of Food and Drug Analysis",

issn = "1021-9498",

publisher = "National Laboratories of Foods and Drugs",

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T1 - A feasible and practical 1H NMR analytical method for the quality control and quantification of bioactive principles in Lycii Fructus

AU - Hsieh, Li Ying

AU - Chan, Hsiu Hui

AU - Kuo, Ping Chung

AU - Hung, Hsin Yi

AU - Li, Yue Chiun

AU - Kuo, Chao Lin

AU - Peng, Yong

AU - Zhao, Zhongzhen

AU - Kuo, Daih Huang

AU - Sun, I. Wen

AU - Wu, Tian Shung

N1 - Funding Information: This study was supported by the Ministry of Science and Technology, Taiwan , to T-S. Wu.

PY - 2018/7

Y1 - 2018/7

N2 - Lycii Fructus, a solanaceous drug, is widely used as functional foods and in Traditional Chinese Medicine. Samples collected from different regions of China have been found to be not identical in chemical compositions which might affect the biological activities. Although many chromatographic and spectrometric methods have been reported to determine the concentration of betaine and other bioactive amino acids, disturbance resulted from other polar substances with low UV-absorbance and expensive mass facilities reduced the applicability of these techniques. In the present study, the strong cation exchange solid phase extraction procedure incorporated with 1H NMR was successfully developed as a rapid and reliable method that can simultaneously determine betaine, citric acid, threonine, alanine, and proline in various Lycii Fructus. In addition, ERETIC 2 method based on PULCON principle was also applied and compared with conventional method. This feasible and practical method offers a very powerful tool for the quality control of commercial Lycii Fructus from different sources.

AB - Lycii Fructus, a solanaceous drug, is widely used as functional foods and in Traditional Chinese Medicine. Samples collected from different regions of China have been found to be not identical in chemical compositions which might affect the biological activities. Although many chromatographic and spectrometric methods have been reported to determine the concentration of betaine and other bioactive amino acids, disturbance resulted from other polar substances with low UV-absorbance and expensive mass facilities reduced the applicability of these techniques. In the present study, the strong cation exchange solid phase extraction procedure incorporated with 1H NMR was successfully developed as a rapid and reliable method that can simultaneously determine betaine, citric acid, threonine, alanine, and proline in various Lycii Fructus. In addition, ERETIC 2 method based on PULCON principle was also applied and compared with conventional method. This feasible and practical method offers a very powerful tool for the quality control of commercial Lycii Fructus from different sources.

KW - H NMR

KW - Betaine

KW - ERETIC 2

KW - Lycii Fructus

KW - PULCON

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U2 - 10.1016/j.jfda.2018.01.001

DO - 10.1016/j.jfda.2018.01.001

M3 - Journal article

C2 - 29976403

AN - SCOPUS:85041172842

SN - 1021-9498

VL - 26

SP - 1105

EP - 1112

JO - Journal of Food and Drug Analysis

JF - Journal of Food and Drug Analysis

IS - 3

ER -

A feasible and practical ¹H NMR analytical method for the quality control and quantification of bioactive principles in Lycii Fructus

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