A feasible and practical 1H NMR analytical method for the quality control and quantification of bioactive principles in Lycii Fructus

Li Ying Hsieh, Hsiu Hui Chan, Ping Chung Kuo, Hsin Yi Hung, Yue Chiun Li, Chao Lin Kuo, Yong Peng, Zhongzhen ZHAO, Daih Huang Kuo, I. Wen Sun, Tian Shung Wu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)


Lycii Fructus, a solanaceous drug, is widely used as functional foods and in Traditional Chinese Medicine. Samples collected from different regions of China have been found to be not identical in chemical compositions which might affect the biological activities. Although many chromatographic and spectrometric methods have been reported to determine the concentration of betaine and other bioactive amino acids, disturbance resulted from other polar substances with low UV-absorbance and expensive mass facilities reduced the applicability of these techniques. In the present study, the strong cation exchange solid phase extraction procedure incorporated with 1H NMR was successfully developed as a rapid and reliable method that can simultaneously determine betaine, citric acid, threonine, alanine, and proline in various Lycii Fructus. In addition, ERETIC 2 method based on PULCON principle was also applied and compared with conventional method. This feasible and practical method offers a very powerful tool for the quality control of commercial Lycii Fructus from different sources.

Original languageEnglish
Pages (from-to)1105-1112
Number of pages8
JournalJournal of Food and Drug Analysis
Issue number3
Publication statusPublished - Jul 2018

Scopus Subject Areas

  • Food Science
  • Pharmacology

User-Defined Keywords

  • H NMR
  • Betaine
  • ERETIC 2
  • Lycii Fructus


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