Abstract
We report a fast fluorescence imaging flow cytometer for phytoplankton analysis that can achieve a volume flow rate up to 1ml/min. The instrument shows a high immunity to motion blur in image captured with a lateral resolution of 0.75 0.06 ?m for a wide size range ~1 ?m to ~200 ?m. This is made possible by suppressing the out-of-focus light using thin light sheet illumination and image deconvolution, and by precluding the motion-blur with a unique flow configuration. Preliminary results from untreated coastal water samples show the technique has high potential as a practical field instrument for monitoring phytoplankton abundance and species composition.
| Original language | English |
|---|---|
| Pages (from-to) | 23921-23926 |
| Number of pages | 6 |
| Journal | Optics Express |
| Volume | 21 |
| Issue number | 20 |
| DOIs | |
| Publication status | Published - 7 Oct 2013 |
Scopus Subject Areas
- Atomic and Molecular Physics, and Optics