A fast fluorescence imaging flow cytometer for phytoplankton analysis

Jianglai Wu, Robert K Y CHAN

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

We report a fast fluorescence imaging flow cytometer for phytoplankton analysis that can achieve a volume flow rate up to 1ml/min. The instrument shows a high immunity to motion blur in image captured with a lateral resolution of 0.75 0.06 ?m for a wide size range ~1 ?m to ~200 ?m. This is made possible by suppressing the out-of-focus light using thin light sheet illumination and image deconvolution, and by precluding the motion-blur with a unique flow configuration. Preliminary results from untreated coastal water samples show the technique has high potential as a practical field instrument for monitoring phytoplankton abundance and species composition.

Original languageEnglish
Pages (from-to)23921-23926
Number of pages6
JournalOptics Express
Volume21
Issue number20
DOIs
Publication statusPublished - 7 Oct 2013

Scopus Subject Areas

  • Atomic and Molecular Physics, and Optics

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