A fast detection of peroxynitrite in living cells

Minglu Li; Xiaojuan Gong; Hung Wing Li; Hui Han; Shaomin Shuang; Shengmei Song; Chuan Dong

doi:10.1016/j.aca.2020.02.009

A fast detection of peroxynitrite in living cells

Minglu Li, Xiaojuan Gong, Hung Wing Li, Hui Han, Shaomin Shuang, Shengmei Song^*, Chuan Dong^*

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

34 Citations (Scopus)

Abstract

Peroxynitrite (ONOO⁻) plays a crucial role in the regulation of diverse pathophysiological processes, and high level of ONOO⁻ is profound association with numerous diseases. Herein, we developed an anthraquinone-based fluorescent probe L for ONOO⁻ determination by a new recognition mechanism: amido oxidized nitroso-group by ONOO⁻. Probe L with amine-based recognition receptor is more selective to ONOO⁻ than other reactive oxygen species, including H₂O₂ and ClO⁻. Furthermore, ONOO⁻ could be rapidly detected by probe L with a Limit of Detection of 13 nM. More importantly, L could be used to monitor intracellular ONOO⁻ in SMMC-7721 cells.

Original language	English
Pages (from-to)	96-102
Number of pages	7
Journal	Analytica Chimica Acta
Volume	1106
DOIs	https://doi.org/10.1016/j.aca.2020.02.009
Publication status	Published - 15 Apr 2020

User-Defined Keywords

Amido oxidized nitroso-group
Fluorescent probe
Peroxynitrite
Reactive oxygen species

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10.1016/j.aca.2020.02.009

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title = "A fast detection of peroxynitrite in living cells",

abstract = "Peroxynitrite (ONOO−) plays a crucial role in the regulation of diverse pathophysiological processes, and high level of ONOO− is profound association with numerous diseases. Herein, we developed an anthraquinone-based fluorescent probe L for ONOO− determination by a new recognition mechanism: amido oxidized nitroso-group by ONOO−. Probe L with amine-based recognition receptor is more selective to ONOO− than other reactive oxygen species, including H2O2 and ClO−. Furthermore, ONOO− could be rapidly detected by probe L with a Limit of Detection of 13 nM. More importantly, L could be used to monitor intracellular ONOO− in SMMC-7721 cells.",

keywords = "Amido oxidized nitroso-group, Fluorescent probe, Peroxynitrite, Reactive oxygen species",

author = "Minglu Li and Xiaojuan Gong and Li, {Hung Wing} and Hui Han and Shaomin Shuang and Shengmei Song and Chuan Dong",

note = "Funding Information: This work was financially supported by the National Natural Science Foundation of China (No. 21575084 and 21705101 ) and the Applied Basic Research Project of Shanxi Province, China (No. 201901D111038 ). Publisher Copyright: {\textcopyright} 2020 Elsevier B.V.",

year = "2020",

month = apr,

day = "15",

doi = "10.1016/j.aca.2020.02.009",

language = "English",

volume = "1106",

pages = "96--102",

journal = "Analytica Chimica Acta",

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T1 - A fast detection of peroxynitrite in living cells

AU - Li, Minglu

AU - Gong, Xiaojuan

AU - Li, Hung Wing

AU - Han, Hui

AU - Shuang, Shaomin

AU - Song, Shengmei

AU - Dong, Chuan

N1 - Funding Information: This work was financially supported by the National Natural Science Foundation of China (No. 21575084 and 21705101 ) and the Applied Basic Research Project of Shanxi Province, China (No. 201901D111038 ). Publisher Copyright: © 2020 Elsevier B.V.

PY - 2020/4/15

Y1 - 2020/4/15

N2 - Peroxynitrite (ONOO−) plays a crucial role in the regulation of diverse pathophysiological processes, and high level of ONOO− is profound association with numerous diseases. Herein, we developed an anthraquinone-based fluorescent probe L for ONOO− determination by a new recognition mechanism: amido oxidized nitroso-group by ONOO−. Probe L with amine-based recognition receptor is more selective to ONOO− than other reactive oxygen species, including H2O2 and ClO−. Furthermore, ONOO− could be rapidly detected by probe L with a Limit of Detection of 13 nM. More importantly, L could be used to monitor intracellular ONOO− in SMMC-7721 cells.

AB - Peroxynitrite (ONOO−) plays a crucial role in the regulation of diverse pathophysiological processes, and high level of ONOO− is profound association with numerous diseases. Herein, we developed an anthraquinone-based fluorescent probe L for ONOO− determination by a new recognition mechanism: amido oxidized nitroso-group by ONOO−. Probe L with amine-based recognition receptor is more selective to ONOO− than other reactive oxygen species, including H2O2 and ClO−. Furthermore, ONOO− could be rapidly detected by probe L with a Limit of Detection of 13 nM. More importantly, L could be used to monitor intracellular ONOO− in SMMC-7721 cells.

KW - Amido oxidized nitroso-group

KW - Fluorescent probe

KW - Peroxynitrite

KW - Reactive oxygen species

UR - http://www.scopus.com/inward/record.url?scp=85079185991&partnerID=8YFLogxK

U2 - 10.1016/j.aca.2020.02.009

DO - 10.1016/j.aca.2020.02.009

M3 - Journal article

C2 - 32145860

AN - SCOPUS:85079185991

SN - 0003-2670

VL - 1106

SP - 96

EP - 102

JO - Analytica Chimica Acta

JF - Analytica Chimica Acta

ER -

A fast detection of peroxynitrite in living cells

Abstract

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