TY - JOUR
T1 - A fast detection of peroxynitrite in living cells
AU - Li, Minglu
AU - Gong, Xiaojuan
AU - Li, Hung Wing
AU - Han, Hui
AU - Shuang, Shaomin
AU - Song, Shengmei
AU - Dong, Chuan
N1 - Funding Information:
This work was financially supported by the National Natural Science Foundation of China (No. 21575084 and 21705101 ) and the Applied Basic Research Project of Shanxi Province, China (No. 201901D111038 ).
Publisher Copyright:
© 2020 Elsevier B.V.
PY - 2020/4/15
Y1 - 2020/4/15
N2 - Peroxynitrite (ONOO−) plays a crucial role in the regulation of diverse pathophysiological processes, and high level of ONOO− is profound association with numerous diseases. Herein, we developed an anthraquinone-based fluorescent probe L for ONOO− determination by a new recognition mechanism: amido oxidized nitroso-group by ONOO−. Probe L with amine-based recognition receptor is more selective to ONOO− than other reactive oxygen species, including H2O2 and ClO−. Furthermore, ONOO− could be rapidly detected by probe L with a Limit of Detection of 13 nM. More importantly, L could be used to monitor intracellular ONOO− in SMMC-7721 cells.
AB - Peroxynitrite (ONOO−) plays a crucial role in the regulation of diverse pathophysiological processes, and high level of ONOO− is profound association with numerous diseases. Herein, we developed an anthraquinone-based fluorescent probe L for ONOO− determination by a new recognition mechanism: amido oxidized nitroso-group by ONOO−. Probe L with amine-based recognition receptor is more selective to ONOO− than other reactive oxygen species, including H2O2 and ClO−. Furthermore, ONOO− could be rapidly detected by probe L with a Limit of Detection of 13 nM. More importantly, L could be used to monitor intracellular ONOO− in SMMC-7721 cells.
KW - Amido oxidized nitroso-group
KW - Fluorescent probe
KW - Peroxynitrite
KW - Reactive oxygen species
UR - http://www.scopus.com/inward/record.url?scp=85079185991&partnerID=8YFLogxK
U2 - 10.1016/j.aca.2020.02.009
DO - 10.1016/j.aca.2020.02.009
M3 - Journal article
C2 - 32145860
AN - SCOPUS:85079185991
SN - 0003-2670
VL - 1106
SP - 96
EP - 102
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
ER -