A dual-functional luminescent probe for imaging H2S in living zebrafish and discrimination hypoxic cells from normoxic cells

Wanhe Wang, Chun Wu, Chao Yang, Guodong Li, Simon Q B HAN, Shengnan Li, Simon Ming Yuen Lee, Chung Hang Leung, Edmond Dik Lung MA*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

In this work, we present a new reaction-based luminogenic probe with dual functions: a highly selective sensor for both in vitro H2S sensing and in vivo H2S imaging in living zebrafish, and a luminescent agent for imaging hypoxic cells. The mechanism of this platform is based on the reduction of the nitro group in the iridium(III) complex to an amino group, which “turns on” its luminescence. Complex 1 exhibited a linear range of detection for H2S from 0 to 170 μM with a detection limit of 4.35 μM. Complex 1 also shows the ability to detect H2S in HeLa cells and in living zebrafish. Meanwhile, complex 1 is capable of discriminating hypoxic from normoxic cells, and shows potential for monitoring hypoxia in zebrafish model.

Original languageEnglish
Pages (from-to)1953-1959
Number of pages7
JournalSensors and Actuators, B: Chemical
Volume255
DOIs
Publication statusPublished - Feb 2018

Scopus Subject Areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

User-Defined Keywords

  • Hydrogen sulfide
  • Iridium(III) complex
  • Luminescence
  • Sensor

Fingerprint

Dive into the research topics of 'A dual-functional luminescent probe for imaging H<sub>2</sub>S in living zebrafish and discrimination hypoxic cells from normoxic cells'. Together they form a unique fingerprint.

Cite this