A DNA microarray for differentiation of the Chinese medicinal herb Dendrobium officinale (Fengdou Shihu) by its 5 S ribosomal DNA intergenic spacer region

Stephen Cho Wing Sze, Kalin Yan Bo Zhang*, Pang Chui Shaw, Paul Pui Hay But, Tzi Bun Ng, Yao Tong*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

A DNA microarray was constructed for high-throughput identification of the plant resource of commercial FDSH [Fengdu Shihu (Dendrobium officinale)]. The 5 S rDNA (ribosomal DNA) intergenic spacer region in D. officinale, D. nobile, D. moniliforme, D. hercoglossum, D. williamsonii, D. capillipes, D. wilsonii and D. jenkinsii was amplified by a single primer pair and sequenced. The sequences showed polymorphism. They were incorporated on a glass slide and hybridized with fluorescently labelled 5 S sequences from commercial Shihu. The DNA microarray enabled the differentiation of D. officinale from the other species tested. FDSH could thus be distinguished from its adulterants. It is evident that DNA microarrays provide a high-throughput and reliable approach for the identification of plant resources, and the method presented here is useful for the authentication of FDSH.
Original languageEnglish
Pages (from-to)149-154
Number of pages6
JournalBiotechnology and Applied Biochemistry
Volume49
Issue number2
Early online date6 Aug 2007
DOIs
Publication statusPublished - Feb 2008
Externally publishedYes

User-Defined Keywords

  • authentification by DNA microarray
  • Chinese medicine
  • Dendrobium officinale (Fengdu Shihu, FDSH
  • identification of plant adulterants
  • 5 S ribosomal DNA gene

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