A DNA microarray for differentiation of the Chinese medicinal herb Dendrobium officinale (Fengdou Shihu) by its 5 S ribosomal DNA intergenic spacer region

Stephen Cho Wing Sze; Kalin Yan Bo Zhang; Pang Chui Shaw; Paul Pui Hay But; Tzi Bun Ng; Yao Tong

doi:10.1042/BA20070059

A DNA microarray for differentiation of the Chinese medicinal herb Dendrobium officinale (Fengdou Shihu) by its 5 S ribosomal DNA intergenic spacer region

Stephen Cho Wing Sze, Kalin Yan Bo Zhang^*, Pang Chui Shaw, Paul Pui Hay But, Tzi Bun Ng, Yao Tong^*

^*Corresponding author for this work

Golden Meditech Centre for NeuroRegeneration Sciences

Research output: Contribution to journal › Journal article › peer-review

17 Citations (Scopus)

Abstract

A DNA microarray was constructed for high-throughput identification of the plant resource of commercial FDSH [Fengdu Shihu (Dendrobium officinale)]. The 5 S rDNA (ribosomal DNA) intergenic spacer region in D. officinale, D. nobile, D. moniliforme, D. hercoglossum, D. williamsonii, D. capillipes, D. wilsonii and D. jenkinsii was amplified by a single primer pair and sequenced. The sequences showed polymorphism. They were incorporated on a glass slide and hybridized with fluorescently labelled 5 S sequences from commercial Shihu. The DNA microarray enabled the differentiation of D. officinale from the other species tested. FDSH could thus be distinguished from its adulterants. It is evident that DNA microarrays provide a high-throughput and reliable approach for the identification of plant resources, and the method presented here is useful for the authentication of FDSH.

Original language	English
Pages (from-to)	149-154
Number of pages	6
Journal	Biotechnology and Applied Biochemistry
Volume	49
Issue number	2
Early online date	6 Aug 2007
DOIs	https://doi.org/10.1042/BA20070059
Publication status	Published - Feb 2008

User-Defined Keywords

authentification by DNA microarray
Chinese medicine
Dendrobium officinale (Fengdu Shihu, FDSH
identification of plant adulterants
5 S ribosomal DNA gene

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10.1042/BA20070059

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title = "A DNA microarray for differentiation of the Chinese medicinal herb Dendrobium officinale (Fengdou Shihu) by its 5 S ribosomal DNA intergenic spacer region",

abstract = "A DNA microarray was constructed for high-throughput identification of the plant resource of commercial FDSH [Fengdu Shihu (Dendrobium officinale)]. The 5 S rDNA (ribosomal DNA) intergenic spacer region in D. officinale, D. nobile, D. moniliforme, D. hercoglossum, D. williamsonii, D. capillipes, D. wilsonii and D. jenkinsii was amplified by a single primer pair and sequenced. The sequences showed polymorphism. They were incorporated on a glass slide and hybridized with fluorescently labelled 5 S sequences from commercial Shihu. The DNA microarray enabled the differentiation of D. officinale from the other species tested. FDSH could thus be distinguished from its adulterants. It is evident that DNA microarrays provide a high-throughput and reliable approach for the identification of plant resources, and the method presented here is useful for the authentication of FDSH.",

keywords = "authentification by DNA microarray, Chinese medicine, Dendrobium officinale (Fengdu Shihu, FDSH, identification of plant adulterants, 5 S ribosomal DNA gene",

author = "Sze, {Stephen Cho Wing} and Zhang, {Kalin Yan Bo} and Shaw, {Pang Chui} and But, {Paul Pui Hay} and Ng, {Tzi Bun} and Yao Tong",

note = "Funding information: This work was supported by a grant (Project Code RGAS076865461) from the University of Hong Kong. We appreciate the help of Professor Zhengtao Wang (Department of Pharmacognosy, Chinese Pharmaceutical University) in providing samples and authentication of plant resources by using morphological and histological methods. Publisher copyright: {\textcopyright}2008 Portland Press Ltd",

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doi = "10.1042/BA20070059",

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volume = "49",

pages = "149--154",

journal = "Biotechnology and Applied Biochemistry",

issn = "0885-4513",

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A DNA microarray for differentiation of the Chinese medicinal herb Dendrobium officinale (Fengdou Shihu) by its 5 S ribosomal DNA intergenic spacer region. / Sze, Stephen Cho Wing; Zhang, Kalin Yan Bo; Shaw, Pang Chui et al.
In: Biotechnology and Applied Biochemistry, Vol. 49, No. 2, 02.2008, p. 149-154.

Research output: Contribution to journal › Journal article › peer-review

TY - JOUR

T1 - A DNA microarray for differentiation of the Chinese medicinal herb Dendrobium officinale (Fengdou Shihu) by its 5 S ribosomal DNA intergenic spacer region

AU - Sze, Stephen Cho Wing

AU - Zhang, Kalin Yan Bo

AU - Shaw, Pang Chui

AU - But, Paul Pui Hay

AU - Ng, Tzi Bun

AU - Tong, Yao

N1 - Funding information: This work was supported by a grant (Project Code RGAS076865461) from the University of Hong Kong. We appreciate the help of Professor Zhengtao Wang (Department of Pharmacognosy, Chinese Pharmaceutical University) in providing samples and authentication of plant resources by using morphological and histological methods. Publisher copyright: ©2008 Portland Press Ltd

PY - 2008/2

Y1 - 2008/2

N2 - A DNA microarray was constructed for high-throughput identification of the plant resource of commercial FDSH [Fengdu Shihu (Dendrobium officinale)]. The 5 S rDNA (ribosomal DNA) intergenic spacer region in D. officinale, D. nobile, D. moniliforme, D. hercoglossum, D. williamsonii, D. capillipes, D. wilsonii and D. jenkinsii was amplified by a single primer pair and sequenced. The sequences showed polymorphism. They were incorporated on a glass slide and hybridized with fluorescently labelled 5 S sequences from commercial Shihu. The DNA microarray enabled the differentiation of D. officinale from the other species tested. FDSH could thus be distinguished from its adulterants. It is evident that DNA microarrays provide a high-throughput and reliable approach for the identification of plant resources, and the method presented here is useful for the authentication of FDSH.

AB - A DNA microarray was constructed for high-throughput identification of the plant resource of commercial FDSH [Fengdu Shihu (Dendrobium officinale)]. The 5 S rDNA (ribosomal DNA) intergenic spacer region in D. officinale, D. nobile, D. moniliforme, D. hercoglossum, D. williamsonii, D. capillipes, D. wilsonii and D. jenkinsii was amplified by a single primer pair and sequenced. The sequences showed polymorphism. They were incorporated on a glass slide and hybridized with fluorescently labelled 5 S sequences from commercial Shihu. The DNA microarray enabled the differentiation of D. officinale from the other species tested. FDSH could thus be distinguished from its adulterants. It is evident that DNA microarrays provide a high-throughput and reliable approach for the identification of plant resources, and the method presented here is useful for the authentication of FDSH.

KW - authentification by DNA microarray

KW - Chinese medicine

KW - Dendrobium officinale (Fengdu Shihu, FDSH

KW - identification of plant adulterants

KW - 5 S ribosomal DNA gene

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DO - 10.1042/BA20070059

M3 - Journal article

SN - 0885-4513

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JO - Biotechnology and Applied Biochemistry

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IS - 2

ER -

A DNA microarray for differentiation of the Chinese medicinal herb Dendrobium officinale (Fengdou Shihu) by its 5 S ribosomal DNA intergenic spacer region

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