A comparative study of void volume markers in immobilized-artificial- membrane and reversed-phase liquid chromatography

Haibin Luo, Yuen Kit CHENG*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

A comparative study of 10 void volume marker candidates on C8, C18, and IAM columns was described under several pH (3.0, 4.8, and 7.3) buffer conditions. Pycnometric analysis has been performed on the columns for comparison. In acidic conditions, small organic carboxylic acids suggested by the manufacturer did not necessarily give reliable and consistent estimates of the void volume for IAM column. However, changes in temperature and organic modifier composition led to small and predictable effects. The results presented here provide minimal but practical guidelines for the use of void volume markers in IAM chromatography.

Original languageEnglish
Pages (from-to)356-361
Number of pages6
JournalJournal of Chromatography A
Volume1103
Issue number2
DOIs
Publication statusPublished - 27 Jan 2006

Scopus Subject Areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

User-Defined Keywords

  • C/C/IAM
  • Inorganic salts
  • Organic acids
  • pK
  • Pycnometry
  • Void volume markers

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