Abstract
A comparative study of 10 void volume marker candidates on C8, C18, and IAM columns was described under several pH (3.0, 4.8, and 7.3) buffer conditions. Pycnometric analysis has been performed on the columns for comparison. In acidic conditions, small organic carboxylic acids suggested by the manufacturer did not necessarily give reliable and consistent estimates of the void volume for IAM column. However, changes in temperature and organic modifier composition led to small and predictable effects. The results presented here provide minimal but practical guidelines for the use of void volume markers in IAM chromatography.
Original language | English |
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Pages (from-to) | 356-361 |
Number of pages | 6 |
Journal | Journal of Chromatography A |
Volume | 1103 |
Issue number | 2 |
DOIs | |
Publication status | Published - 27 Jan 2006 |
Scopus Subject Areas
- Analytical Chemistry
- Biochemistry
- Organic Chemistry
User-Defined Keywords
- C/C/IAM
- Inorganic salts
- Organic acids
- pK
- Pycnometry
- Void volume markers