A chromosome-level genome assembly of anesthetic drug–producing Anisodus acutangulus provides insights into its evolution and the biosynthesis of tropane alkaloids

Wei Zhou, Can Wang, Xiaolong Hao, Fei Chen, Qikai Huang, Tingyao Liu, Jiang Xu, Shuai Guo, Baosheng Liao, Zhixiang Liu, Yue Feng, Yao Wang, Pan Liao, Jiayu Xue, Min Shi, Itay Maoz, Guoyin Kai*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

2 Citations (Scopus)

Abstract

Tropane alkaloids (TAs), which are anticholinergic agents, are an essential class of natural compounds, and there is a growing demand for TAs with anesthetic, analgesic, and spasmolytic effects. Anisodus acutangulus (Solanaceae) is a TA-producing plant that was used as an anesthetic in ancient China. In this study, we assembled a high-quality, chromosome-scale genome of A. acutangulus with a contig N50 of 7.4 Mb. A recent whole-genome duplication occurred in A. acutangulus after its divergence from other Solanaceae species, which resulted in the duplication of ADC1 and UGT genes involved in TA biosynthesis. The catalytic activities of H6H enzymes were determined for three Solanaceae plants. On the basis of evolution and co-expressed genes, AaWRKY11 was selected for further analyses, which revealed that its encoded transcription factor promotes TA biosynthesis by activating AaH6H1 expression. These findings provide useful insights into genome evolution related to TA biosynthesis and have potential implications for genetic manipulation of TA-producing plants.

Original languageEnglish
Article number100680
Number of pages14
JournalPlant Communications
Volume5
Issue number1
DOIs
Publication statusPublished - 8 Jan 2024

Scopus Subject Areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Plant Science
  • Cell Biology

User-Defined Keywords

  • AaWRKY11
  • Anisodus acutangulus
  • biosynthesis
  • hyoscyamine 6 β-hydroxylase
  • tropane alkaloid

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