A bright and responsive europium probe for determination of pH change within the endoplasmic reticulum of living cells

Brian K. Mc Mahon; Robert Pal; David Parker

doi:10.1039/c3cc42308e

A bright and responsive europium probe for determination of pH change within the endoplasmic reticulum of living cells

Brian K. Mc Mahon, Robert Pal, David Parker^*

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

113 Citations (Scopus)

Abstract

A ratiometric Eu^III complex has been developed that localises selectively within the endoplasmic reticulum of living cells. Careful calibration, using a time-gated spectral imaging microscope, allows the intensity ratio of emission bands and the variation of excited state lifetime to be used for pH determination, with a pK _a of 7.15.

Original language	English
Pages (from-to)	5363-5365
Number of pages	3
Journal	Chemical Communications
Volume	49
Issue number	47
Early online date	1 May 2013
DOIs	https://doi.org/10.1039/c3cc42308e
Publication status	Published - 14 Jun 2013

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10.1039/c3cc42308e

Cite this

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title = "A bright and responsive europium probe for determination of pH change within the endoplasmic reticulum of living cells",

abstract = "A ratiometric EuIII complex has been developed that localises selectively within the endoplasmic reticulum of living cells. Careful calibration, using a time-gated spectral imaging microscope, allows the intensity ratio of emission bands and the variation of excited state lifetime to be used for pH determination, with a pK a of 7.15.",

author = "{Mc Mahon}, {Brian K.} and Robert Pal and David Parker",

note = "We thank the ERC (FCC 266804) for support. Publisher Copyright: {\textcopyright} The Royal Society of Chemistry 2013",

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T1 - A bright and responsive europium probe for determination of pH change within the endoplasmic reticulum of living cells

AU - Mc Mahon, Brian K.

AU - Pal, Robert

AU - Parker, David

N1 - We thank the ERC (FCC 266804) for support. Publisher Copyright: © The Royal Society of Chemistry 2013

PY - 2013/6/14

Y1 - 2013/6/14

N2 - A ratiometric EuIII complex has been developed that localises selectively within the endoplasmic reticulum of living cells. Careful calibration, using a time-gated spectral imaging microscope, allows the intensity ratio of emission bands and the variation of excited state lifetime to be used for pH determination, with a pK a of 7.15.

AB - A ratiometric EuIII complex has been developed that localises selectively within the endoplasmic reticulum of living cells. Careful calibration, using a time-gated spectral imaging microscope, allows the intensity ratio of emission bands and the variation of excited state lifetime to be used for pH determination, with a pK a of 7.15.

UR - http://www.scopus.com/inward/record.url?scp=84878076981&partnerID=8YFLogxK

UR - https://pubs.rsc.org/en/content/articlelanding/2013/cc/c3cc42308e

U2 - 10.1039/c3cc42308e

DO - 10.1039/c3cc42308e

M3 - Journal article

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AN - SCOPUS:84878076981

SN - 1359-7345

VL - 49

SP - 5363

EP - 5365

JO - Chemical Communications

JF - Chemical Communications

IS - 47

ER -

A bright and responsive europium probe for determination of pH change within the endoplasmic reticulum of living cells

Abstract

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