A bioorthogonal time-resolved luminogenic probe for metabolic labelling and imaging of glycans

Judun Zheng, Qiuqiang Zhan, Lijun Jiang, Da Xing*, Tao Zhang*, Ka-Leung Wong*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

5 Citations (Scopus)

Abstract

The use of bioorthogonal fluorogenic probes is superior to labelling and imaging of biomolecules in live cells and organisms, although overcoming the limitation of autofluorescence is still a challenge for current probes to achieve high illumination resolution of the target of interest. We herein demonstrate a functionalized terbium complex Tb-1 that is stable and biocompatible to enable bioorthogonal ligation with engineered cell-surface glycans for providing responsive luminescence. A luminescence resonance energy transfer (LRET) quencher with bioorthogonal properties is strategically incorporated into a tripodal terbium complex with low toxicity, which can undergo a click-cycloaddition reaction with a cyclooctene to completely change the electronic structure of the quencher, resulting in a much less efficient LRET but a 5-fold enhancement in the long-lived terbium emission intensity. This work therefore establishes a time-resolved platform that enables labelling and imaging of the biomolecules of interest. This journal is

Original languageEnglish
Pages (from-to)4062-4069
Number of pages8
JournalInorganic Chemistry Frontiers
Volume7
Issue number21
DOIs
Publication statusPublished - 7 Nov 2020

Scopus Subject Areas

  • Inorganic Chemistry

Fingerprint

Dive into the research topics of 'A bioorthogonal time-resolved luminogenic probe for metabolic labelling and imaging of glycans'. Together they form a unique fingerprint.

Cite this