A bioaccumulative cyclometalated platinum(II) complex with two-photon-induced emission for live cell imaging

Chi Kin Koo; Ka-Leung Wong; Cornelia Wing Yin Man; Yun Wah Lam; Leo King Yan So; Hoi Lam Tam; Sai Wah Tsao; Kok Wai Cheah; Kai Chung Lau; Yang Yi Yang; Jin Can Chen; Michael Hon Wah Lam

doi:10.1021/ic801261h

A bioaccumulative cyclometalated platinum(II) complex with two-photon-induced emission for live cell imaging

Chi Kin Koo
, Ka-Leung Wong
, Cornelia Wing Yin Man
, Yun Wah Lam
, Leo King Yan So
, Hoi Lam Tam
, Sai Wah Tsao
, Kok Wai Cheah
, Kai Chung Lau
, Yang Yi Yang
, Jin Can Chen
, Michael Hon Wah Lam

Research output: Contribution to journal › Journal article › peer-review

97 Citations (Scopus)

Abstract

The cyclometalated platinum(II) complex [Pt(L)Cl], where HL is a new cyclometalating ligand 2-phenyl-6-(1H-pyrazol-3-yl)pyridine containing C _phenyl, N_pyridyl, and N_pyrazolyl donor moieties, was found to possess two-photon-induced luminescent properties. The two-photon-absorption cross section of the complex in N,N-dimethylformamide at room temperature was measured to be 20.8 GM. Upon two-photon excitation at 730 nm from a Ti:sapphire laser, bright-green emission was observed. Besides its two-photon-induced luminescent properties, [Pt(L)Cl] was able to be rapidly accumulated in live HeLa and NIH3T3 cells. The two-photon-induced luminescence of the complex was retained after live cell internalization and can be observed by two-photon confocal microscopy. Its bioaccumulation properties enabled time-lapse imaging of the internalization process of the dye into living cells. Cytotoxicity of [Pt(L)Cl] to both tested cell lines was low, according to MTT assays, even at loadings as high as 20 times the dose concentration for imaging for 6 h.

Original language	English
Pages (from-to)	872-878
Number of pages	7
Journal	Inorganic Chemistry
Volume	48
Issue number	3
DOIs	https://doi.org/10.1021/ic801261h
Publication status	Published - 2 Feb 2009

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10.1021/ic801261h

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@article{050f7624fe724257a8725eed7957927f,

title = "A bioaccumulative cyclometalated platinum(II) complex with two-photon-induced emission for live cell imaging",

abstract = "The cyclometalated platinum(II) complex [Pt(L)Cl], where HL is a new cyclometalating ligand 2-phenyl-6-(1H-pyrazol-3-yl)pyridine containing C phenyl, Npyridyl, and Npyrazolyl donor moieties, was found to possess two-photon-induced luminescent properties. The two-photon-absorption cross section of the complex in N,N-dimethylformamide at room temperature was measured to be 20.8 GM. Upon two-photon excitation at 730 nm from a Ti:sapphire laser, bright-green emission was observed. Besides its two-photon-induced luminescent properties, [Pt(L)Cl] was able to be rapidly accumulated in live HeLa and NIH3T3 cells. The two-photon-induced luminescence of the complex was retained after live cell internalization and can be observed by two-photon confocal microscopy. Its bioaccumulation properties enabled time-lapse imaging of the internalization process of the dye into living cells. Cytotoxicity of [Pt(L)Cl] to both tested cell lines was low, according to MTT assays, even at loadings as high as 20 times the dose concentration for imaging for 6 h.",

author = "Koo, \{Chi Kin\} and Ka-Leung Wong and Man, \{Cornelia Wing Yin\} and Lam, \{Yun Wah\} and So, \{Leo King Yan\} and Tam, \{Hoi Lam\} and Tsao, \{Sai Wah\} and Cheah, \{Kok Wai\} and Lau, \{Kai Chung\} and Yang, \{Yang Yi\} and Chen, \{Jin Can\} and Lam, \{Michael Hon Wah\}",

note = "Funding information: The authors acknowledge financial support from the Hong Kong Research Grants Council, City University of Hong Kong, and Hong Kong Baptist University. K.-L.W. acknowledges a Research Scholarship Enhancement Scheme from City University of Hong Kong. Publisher copyright: {\textcopyright} 2009 American Chemical Society",

year = "2009",

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doi = "10.1021/ic801261h",

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T1 - A bioaccumulative cyclometalated platinum(II) complex with two-photon-induced emission for live cell imaging

AU - Koo, Chi Kin

AU - Wong, Ka-Leung

AU - Man, Cornelia Wing Yin

AU - Lam, Yun Wah

AU - So, Leo King Yan

AU - Tam, Hoi Lam

AU - Tsao, Sai Wah

AU - Cheah, Kok Wai

AU - Lau, Kai Chung

AU - Yang, Yang Yi

AU - Chen, Jin Can

AU - Lam, Michael Hon Wah

N1 - Funding information: The authors acknowledge financial support from the Hong Kong Research Grants Council, City University of Hong Kong, and Hong Kong Baptist University. K.-L.W. acknowledges a Research Scholarship Enhancement Scheme from City University of Hong Kong. Publisher copyright: © 2009 American Chemical Society

PY - 2009/2/2

Y1 - 2009/2/2

N2 - The cyclometalated platinum(II) complex [Pt(L)Cl], where HL is a new cyclometalating ligand 2-phenyl-6-(1H-pyrazol-3-yl)pyridine containing C phenyl, Npyridyl, and Npyrazolyl donor moieties, was found to possess two-photon-induced luminescent properties. The two-photon-absorption cross section of the complex in N,N-dimethylformamide at room temperature was measured to be 20.8 GM. Upon two-photon excitation at 730 nm from a Ti:sapphire laser, bright-green emission was observed. Besides its two-photon-induced luminescent properties, [Pt(L)Cl] was able to be rapidly accumulated in live HeLa and NIH3T3 cells. The two-photon-induced luminescence of the complex was retained after live cell internalization and can be observed by two-photon confocal microscopy. Its bioaccumulation properties enabled time-lapse imaging of the internalization process of the dye into living cells. Cytotoxicity of [Pt(L)Cl] to both tested cell lines was low, according to MTT assays, even at loadings as high as 20 times the dose concentration for imaging for 6 h.

AB - The cyclometalated platinum(II) complex [Pt(L)Cl], where HL is a new cyclometalating ligand 2-phenyl-6-(1H-pyrazol-3-yl)pyridine containing C phenyl, Npyridyl, and Npyrazolyl donor moieties, was found to possess two-photon-induced luminescent properties. The two-photon-absorption cross section of the complex in N,N-dimethylformamide at room temperature was measured to be 20.8 GM. Upon two-photon excitation at 730 nm from a Ti:sapphire laser, bright-green emission was observed. Besides its two-photon-induced luminescent properties, [Pt(L)Cl] was able to be rapidly accumulated in live HeLa and NIH3T3 cells. The two-photon-induced luminescence of the complex was retained after live cell internalization and can be observed by two-photon confocal microscopy. Its bioaccumulation properties enabled time-lapse imaging of the internalization process of the dye into living cells. Cytotoxicity of [Pt(L)Cl] to both tested cell lines was low, according to MTT assays, even at loadings as high as 20 times the dose concentration for imaging for 6 h.

UR - https://www.scopus.com/pages/publications/60849111428

U2 - 10.1021/ic801261h

DO - 10.1021/ic801261h

M3 - Journal article

C2 - 19128152

AN - SCOPUS:60849111428

SN - 0020-1669

VL - 48

SP - 872

EP - 878

JO - Inorganic Chemistry

JF - Inorganic Chemistry

IS - 3

ER -

A bioaccumulative cyclometalated platinum(II) complex with two-photon-induced emission for live cell imaging

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