A bioaccumulative cyclometalated platinum(II) complex with two-photon-induced emission for live cell imaging

Chi Kin Koo; Ka-Leung Wong; Cornelia Wing Yin Man; Yun Wah Lam; Leo King Yan So; Hoi Lam Tam; Sai Wah Tsao; Kok Wai Cheah; Kai Chung Lau; Yang Yi Yang; Jin Can Chen; Michael Hon Wah Lam

doi:10.1021/ic801261h

A bioaccumulative cyclometalated platinum(II) complex with two-photon-induced emission for live cell imaging

Chi Kin Koo, Ka-Leung Wong, Cornelia Wing Yin Man, Yun Wah Lam, Leo King Yan So, Hoi Lam Tam, Sai Wah Tsao, Kok Wai Cheah, Kai Chung Lau, Yang Yi Yang, Jin Can Chen, Michael Hon Wah Lam

Research output: Contribution to journal › Journal article › peer-review

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Abstract

The cyclometalated platinum(II) complex [Pt(L)Cl], where HL is a new cyclometalating ligand 2-phenyl-6-(1H-pyrazol-3-yl)pyridine containing C _phenyl, N_pyridyl, and N_pyrazolyl donor moieties, was found to possess two-photon-induced luminescent properties. The two-photon-absorption cross section of the complex in N,N-dimethylformamide at room temperature was measured to be 20.8 GM. Upon two-photon excitation at 730 nm from a Ti:sapphire laser, bright-green emission was observed. Besides its two-photon-induced luminescent properties, [Pt(L)Cl] was able to be rapidly accumulated in live HeLa and NIH3T3 cells. The two-photon-induced luminescence of the complex was retained after live cell internalization and can be observed by two-photon confocal microscopy. Its bioaccumulation properties enabled time-lapse imaging of the internalization process of the dye into living cells. Cytotoxicity of [Pt(L)Cl] to both tested cell lines was low, according to MTT assays, even at loadings as high as 20 times the dose concentration for imaging for 6 h.

Original language	English
Pages (from-to)	872-878
Number of pages	7
Journal	Inorganic Chemistry
Volume	48
Issue number	3
DOIs	https://doi.org/10.1021/ic801261h
Publication status	Published - 2 Feb 2009

Scopus Subject Areas

Physical and Theoretical Chemistry
Inorganic Chemistry

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@article{050f7624fe724257a8725eed7957927f,

title = "A bioaccumulative cyclometalated platinum(II) complex with two-photon-induced emission for live cell imaging",

abstract = "The cyclometalated platinum(II) complex [Pt(L)Cl], where HL is a new cyclometalating ligand 2-phenyl-6-(1H-pyrazol-3-yl)pyridine containing C phenyl, Npyridyl, and Npyrazolyl donor moieties, was found to possess two-photon-induced luminescent properties. The two-photon-absorption cross section of the complex in N,N-dimethylformamide at room temperature was measured to be 20.8 GM. Upon two-photon excitation at 730 nm from a Ti:sapphire laser, bright-green emission was observed. Besides its two-photon-induced luminescent properties, [Pt(L)Cl] was able to be rapidly accumulated in live HeLa and NIH3T3 cells. The two-photon-induced luminescence of the complex was retained after live cell internalization and can be observed by two-photon confocal microscopy. Its bioaccumulation properties enabled time-lapse imaging of the internalization process of the dye into living cells. Cytotoxicity of [Pt(L)Cl] to both tested cell lines was low, according to MTT assays, even at loadings as high as 20 times the dose concentration for imaging for 6 h.",

author = "Koo, {Chi Kin} and Ka-Leung Wong and Man, {Cornelia Wing Yin} and Lam, {Yun Wah} and So, {Leo King Yan} and Tam, {Hoi Lam} and Tsao, {Sai Wah} and Cheah, {Kok Wai} and Lau, {Kai Chung} and Yang, {Yang Yi} and Chen, {Jin Can} and Lam, {Michael Hon Wah}",

note = "Funding information: The authors acknowledge financial support from the Hong Kong Research Grants Council, City University of Hong Kong, and Hong Kong Baptist University. K.-L.W. acknowledges a Research Scholarship Enhancement Scheme from City University of Hong Kong. Publisher copyright: {\textcopyright} 2009 American Chemical Society",

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doi = "10.1021/ic801261h",

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T1 - A bioaccumulative cyclometalated platinum(II) complex with two-photon-induced emission for live cell imaging

AU - Koo, Chi Kin

AU - Wong, Ka-Leung

AU - Man, Cornelia Wing Yin

AU - Lam, Yun Wah

AU - So, Leo King Yan

AU - Tam, Hoi Lam

AU - Tsao, Sai Wah

AU - Cheah, Kok Wai

AU - Lau, Kai Chung

AU - Yang, Yang Yi

AU - Chen, Jin Can

AU - Lam, Michael Hon Wah

N1 - Funding information: The authors acknowledge financial support from the Hong Kong Research Grants Council, City University of Hong Kong, and Hong Kong Baptist University. K.-L.W. acknowledges a Research Scholarship Enhancement Scheme from City University of Hong Kong. Publisher copyright: © 2009 American Chemical Society

PY - 2009/2/2

Y1 - 2009/2/2

N2 - The cyclometalated platinum(II) complex [Pt(L)Cl], where HL is a new cyclometalating ligand 2-phenyl-6-(1H-pyrazol-3-yl)pyridine containing C phenyl, Npyridyl, and Npyrazolyl donor moieties, was found to possess two-photon-induced luminescent properties. The two-photon-absorption cross section of the complex in N,N-dimethylformamide at room temperature was measured to be 20.8 GM. Upon two-photon excitation at 730 nm from a Ti:sapphire laser, bright-green emission was observed. Besides its two-photon-induced luminescent properties, [Pt(L)Cl] was able to be rapidly accumulated in live HeLa and NIH3T3 cells. The two-photon-induced luminescence of the complex was retained after live cell internalization and can be observed by two-photon confocal microscopy. Its bioaccumulation properties enabled time-lapse imaging of the internalization process of the dye into living cells. Cytotoxicity of [Pt(L)Cl] to both tested cell lines was low, according to MTT assays, even at loadings as high as 20 times the dose concentration for imaging for 6 h.

AB - The cyclometalated platinum(II) complex [Pt(L)Cl], where HL is a new cyclometalating ligand 2-phenyl-6-(1H-pyrazol-3-yl)pyridine containing C phenyl, Npyridyl, and Npyrazolyl donor moieties, was found to possess two-photon-induced luminescent properties. The two-photon-absorption cross section of the complex in N,N-dimethylformamide at room temperature was measured to be 20.8 GM. Upon two-photon excitation at 730 nm from a Ti:sapphire laser, bright-green emission was observed. Besides its two-photon-induced luminescent properties, [Pt(L)Cl] was able to be rapidly accumulated in live HeLa and NIH3T3 cells. The two-photon-induced luminescence of the complex was retained after live cell internalization and can be observed by two-photon confocal microscopy. Its bioaccumulation properties enabled time-lapse imaging of the internalization process of the dye into living cells. Cytotoxicity of [Pt(L)Cl] to both tested cell lines was low, according to MTT assays, even at loadings as high as 20 times the dose concentration for imaging for 6 h.

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A bioaccumulative cyclometalated platinum(II) complex with two-photon-induced emission for live cell imaging

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