A 2,7-carbazole-based dicationic salt for fluorescence detection of nucleic acids and two-photon fluorescence imaging of RNA in nucleoli and cytoplasm

Xin Liu; Yuming Sun; Yuanhong Zhang; Fang Miao; Guancong Wang; Hongshi Zhao; Xiaoqiang Yu; Hong Liu; Wai Yeung Wong

doi:10.1039/c1ob05123g

A 2,7-carbazole-based dicationic salt for fluorescence detection of nucleic acids and two-photon fluorescence imaging of RNA in nucleoli and cytoplasm

Xin Liu
, Yuming Sun
, Yuanhong Zhang
, Fang Miao
, Guancong Wang
, Hongshi Zhao
, Xiaoqiang Yu^*
, Hong Liu
, Wai Yeung Wong

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

75 Citations (Scopus)

Abstract

A new carbazole-derived dicationic compound, namely 2,7-bis(1-hydroxyethyl- 4-vinylpyridinium iodine)-N-ethylcarbazole (2,7-9E-BHVC), with a large two-photon action absorption cross section in nucleic acids has been obtained. Moreover, it possesses the potential of imaging RNA in nucleoli and cytoplasm in two-photon fluorescence microscopy and exhibits good counterstain compatibility with the commercial fluorescent nucleic dye DAPI.

Original language	English
Pages (from-to)	3615-3618
Number of pages	4
Journal	Organic and Biomolecular Chemistry
Volume	9
Issue number	10
DOIs	https://doi.org/10.1039/c1ob05123g
Publication status	Published - 21 May 2011

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10.1039/c1ob05123g

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title = "A 2,7-carbazole-based dicationic salt for fluorescence detection of nucleic acids and two-photon fluorescence imaging of RNA in nucleoli and cytoplasm",

abstract = "A new carbazole-derived dicationic compound, namely 2,7-bis(1-hydroxyethyl- 4-vinylpyridinium iodine)-N-ethylcarbazole (2,7-9E-BHVC), with a large two-photon action absorption cross section in nucleic acids has been obtained. Moreover, it possesses the potential of imaging RNA in nucleoli and cytoplasm in two-photon fluorescence microscopy and exhibits good counterstain compatibility with the commercial fluorescent nucleic dye DAPI.",

author = "Xin Liu and Yuming Sun and Yuanhong Zhang and Fang Miao and Guancong Wang and Hongshi Zhao and Xiaoqiang Yu and Hong Liu and Wong, \{Wai Yeung\}",

note = "the National Science Foundation of China (50673053, 50173015, 30771091, 50990061 and 50721002), NSFC/RGC (50218001), Open Point of State Key Laboratory of Supramolecular Structure and Materials and Innovation Fund for the Post-Doctoral Program of Shandong Province (201003076). W.-Y.W. also acknowledges financial support from the University Grants Committee of HKSAR, China (Project No. [AoE/P-03/08]) and Hong Kong Baptist University (FRG/08-09/I-20).",

year = "2011",

month = may,

day = "21",

doi = "10.1039/c1ob05123g",

language = "English",

volume = "9",

pages = "3615--3618",

journal = "Organic and Biomolecular Chemistry",

issn = "1477-0520",

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TY - JOUR

T1 - A 2,7-carbazole-based dicationic salt for fluorescence detection of nucleic acids and two-photon fluorescence imaging of RNA in nucleoli and cytoplasm

AU - Liu, Xin

AU - Sun, Yuming

AU - Zhang, Yuanhong

AU - Miao, Fang

AU - Wang, Guancong

AU - Zhao, Hongshi

AU - Yu, Xiaoqiang

AU - Liu, Hong

AU - Wong, Wai Yeung

N1 - the National Science Foundation of China (50673053, 50173015, 30771091, 50990061 and 50721002), NSFC/RGC (50218001), Open Point of State Key Laboratory of Supramolecular Structure and Materials and Innovation Fund for the Post-Doctoral Program of Shandong Province (201003076). W.-Y.W. also acknowledges financial support from the University Grants Committee of HKSAR, China (Project No. [AoE/P-03/08]) and Hong Kong Baptist University (FRG/08-09/I-20).

PY - 2011/5/21

Y1 - 2011/5/21

N2 - A new carbazole-derived dicationic compound, namely 2,7-bis(1-hydroxyethyl- 4-vinylpyridinium iodine)-N-ethylcarbazole (2,7-9E-BHVC), with a large two-photon action absorption cross section in nucleic acids has been obtained. Moreover, it possesses the potential of imaging RNA in nucleoli and cytoplasm in two-photon fluorescence microscopy and exhibits good counterstain compatibility with the commercial fluorescent nucleic dye DAPI.

AB - A new carbazole-derived dicationic compound, namely 2,7-bis(1-hydroxyethyl- 4-vinylpyridinium iodine)-N-ethylcarbazole (2,7-9E-BHVC), with a large two-photon action absorption cross section in nucleic acids has been obtained. Moreover, it possesses the potential of imaging RNA in nucleoli and cytoplasm in two-photon fluorescence microscopy and exhibits good counterstain compatibility with the commercial fluorescent nucleic dye DAPI.

UR - https://www.scopus.com/pages/publications/79955376861

U2 - 10.1039/c1ob05123g

DO - 10.1039/c1ob05123g

M3 - Journal article

C2 - 21431187

AN - SCOPUS:79955376861

SN - 1477-0520

VL - 9

SP - 3615

EP - 3618

JO - Organic and Biomolecular Chemistry

JF - Organic and Biomolecular Chemistry

IS - 10

ER -

A 2,7-carbazole-based dicationic salt for fluorescence detection of nucleic acids and two-photon fluorescence imaging of RNA in nucleoli and cytoplasm

Abstract

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