TY - JOUR
T1 - 3-Acetylpyridine On-Tissue Paternò–Büchi Derivatization Enabling High Coverage Lipid C═C Location-Resolved MS Imaging in Biological Tissues
AU - Chen, Yanyan
AU - Xie, Chengyi
AU - Wang, Xiaoxiao
AU - Cao, Guodong
AU - Ru, Yi
AU - Song, Yuanyuan
AU - Iyaswamy, Ashok
AU - Li, Min
AU - Wang, Jianing
AU - Cai, Zongwei
N1 - Funding Information:
This work was supported by the Start-up Grant from Hong Kong Baptist University, SKLEBA Research Grant (SKLP_2021_P04), the Shenzhen Science and Technology Innovation Commission (2021Szvup134), the grants of SKELBA (HKBU-SKLP_2021_P05), the Health and Medical Research Fund (HMRF/17182541, HMRF/17182551, and HMRF/19200721), and the research grants from Hong Kong Baptist University (HKBU/RC-IRCs/17-18/03 and IRCMS/19-20/H02). We would like to thank Dr. Simon Wang for his help in editing the manuscript and Dr. Xiaobo Xie, Analytical & Testing Center, Sichuan University, for her help in the PB reaction experiment.
Publisher Copyright:
© 2022 American Chemical Society
PY - 2022/11/8
Y1 - 2022/11/8
N2 - Unsaturated lipids containing single or more carbon–carbon double bonds (C═C) within tissues are closely associated with various types of diseases. Mass spectrometry imaging (MSI) has been used to study the spatial distribution of lipid C═C location isomers in tissue sections. However, comprehensive characterization of lipid C═C location isomers using MSI remains challenging. Herein, we established an on-tissue charge-switching Paternò–Büchi (PB) derivatization method using 3-acetylpyridine (3-AP) as a reaction reagent, which can be used to detect and assign C═C location of glycerophospholipids (GPLs) as well as neutral lipids, such as fatty acids (FAs), under the same experimental workflow using matrix-assisted laser desorption/ionization (MALDI)-MSI. High coverage of mono- and poly-unsaturated C═C location isomers among various lipid classes including FA, phosphatidylcholine (PC), and sulfatide (SHexCer) in distinct regions of the mouse brain and kidney was visualized using MALDI-MS/MS imaging. This method has also been applied to map the spatial distribution of lipid C═C location isomers in the Alzheimer’s disease (AD) mice model for the first time, which provides a new tool to study the relationships between the distribution of lipid structural diversity and neurodegenerative diseases.
AB - Unsaturated lipids containing single or more carbon–carbon double bonds (C═C) within tissues are closely associated with various types of diseases. Mass spectrometry imaging (MSI) has been used to study the spatial distribution of lipid C═C location isomers in tissue sections. However, comprehensive characterization of lipid C═C location isomers using MSI remains challenging. Herein, we established an on-tissue charge-switching Paternò–Büchi (PB) derivatization method using 3-acetylpyridine (3-AP) as a reaction reagent, which can be used to detect and assign C═C location of glycerophospholipids (GPLs) as well as neutral lipids, such as fatty acids (FAs), under the same experimental workflow using matrix-assisted laser desorption/ionization (MALDI)-MSI. High coverage of mono- and poly-unsaturated C═C location isomers among various lipid classes including FA, phosphatidylcholine (PC), and sulfatide (SHexCer) in distinct regions of the mouse brain and kidney was visualized using MALDI-MS/MS imaging. This method has also been applied to map the spatial distribution of lipid C═C location isomers in the Alzheimer’s disease (AD) mice model for the first time, which provides a new tool to study the relationships between the distribution of lipid structural diversity and neurodegenerative diseases.
UR - http://www.scopus.com/inward/record.url?scp=85141035783&partnerID=8YFLogxK
U2 - 10.1021/acs.analchem.2c03089
DO - 10.1021/acs.analchem.2c03089
M3 - Journal article
SN - 0003-2700
VL - 94
SP - 15367
EP - 15376
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 44
ER -