3-Acetylpyridine On-Tissue Paternò–Büchi Derivatization Enabling High Coverage Lipid C═C Location-Resolved MS Imaging in Biological Tissues

Yanyan Chen, Chengyi Xie, Xiaoxiao Wang, Guodong Cao, Yi Ru, Yuanyuan Song, Ashok Iyaswamy, Min Li, Jianing Wang*, Zongwei Cai*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

15 Citations (Scopus)

Abstract

Unsaturated lipids containing single or more carbon–carbon double bonds (C═C) within tissues are closely associated with various types of diseases. Mass spectrometry imaging (MSI) has been used to study the spatial distribution of lipid C═C location isomers in tissue sections. However, comprehensive characterization of lipid C═C location isomers using MSI remains challenging. Herein, we established an on-tissue charge-switching Paternò–Büchi (PB) derivatization method using 3-acetylpyridine (3-AP) as a reaction reagent, which can be used to detect and assign C═C location of glycerophospholipids (GPLs) as well as neutral lipids, such as fatty acids (FAs), under the same experimental workflow using matrix-assisted laser desorption/ionization (MALDI)-MSI. High coverage of mono- and poly-unsaturated C═C location isomers among various lipid classes including FA, phosphatidylcholine (PC), and sulfatide (SHexCer) in distinct regions of the mouse brain and kidney was visualized using MALDI-MS/MS imaging. This method has also been applied to map the spatial distribution of lipid C═C location isomers in the Alzheimer’s disease (AD) mice model for the first time, which provides a new tool to study the relationships between the distribution of lipid structural diversity and neurodegenerative diseases.

Original languageEnglish
Pages (from-to)15367-15376
Number of pages10
JournalAnalytical Chemistry
Volume94
Issue number44
Early online date26 Oct 2022
DOIs
Publication statusPublished - 8 Nov 2022

Scopus Subject Areas

  • Analytical Chemistry

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