Abstract
采用肝微粒体体外温孵法,对2,3,7,8-TCDD在大鼠肝微粒体中进行体外代谢研究，利用实验中所建立的液相色谱串联质谱方法对其代谢产物进行直接分析，共鉴别出两类代谢产物，分别为2个单羟基化代谢产物(2-OH-1,3,7,8-TCDD和1-OH-2,3,7,8-TCDD)和双羟基化脱氯的代谢产物。
2,3,7,8-TCDD is potentially harmflu to human and animal health. The aim of this study is to identify metabolites of 2,3,7,8-TCDD from the in vitro metabolism using rat liver microsome P450 enzymes. We had indentified two kinds of metabolites of 2,3,7,8-TCDD by using liquid chromatography coupled with tandem mass spectrometry,namely,monohydroxylated metabolites of 2,3,7,8-TCDD and trichloro-dihydroxydibenzo-p-dioxins.
2,3,7,8-TCDD is potentially harmflu to human and animal health. The aim of this study is to identify metabolites of 2,3,7,8-TCDD from the in vitro metabolism using rat liver microsome P450 enzymes. We had indentified two kinds of metabolites of 2,3,7,8-TCDD by using liquid chromatography coupled with tandem mass spectrometry,namely,monohydroxylated metabolites of 2,3,7,8-TCDD and trichloro-dihydroxydibenzo-p-dioxins.
| Translated title of the contribution | Analysis of in vitro metabolites of 2,3,7,8-TCDD in rat liver microsomes by LC-ESI-MS/MS |
|---|---|
| Original language | Chinese (Simplified) |
| Pages (from-to) | 1353-1357 |
| Number of pages | 5 |
| Journal | 环境化学 |
| Volume | 32 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - Jul 2013 |
User-Defined Keywords
- 2,3,7,8-TCDD
- 液质联用(LC-MS/MS)
- 大鼠肝微粒体
- 体外代谢
- LC-MS/MS
- rat liver microsomes
- in vitro metabolite
