黄芪多糖的提取及精制工艺研究

目的: 优化黄芪多糖的提取及精制工艺。方法:以粗多糖提取率和葡萄糖计多糖得率为指标,采用正交试验确定水提醇沉法最佳工艺条件; 以多糖损失率、蛋白脱除率、色素脱除率结合可见光区光谱曲线下面积为测定指标, 考察不同方法除蛋白、脱色素的效果以及对黄芪多糖含量的影响。结果: 8倍量水在100℃下提取90 min, 共提取3次为最佳提取条件;胃蛋白酶结合Sevage法除蛋白、DE52脱色素为最佳精制工艺。结论:该提取、精制工艺简便、快捷、高效, 为黄芪多糖的进一步研究提供了基础。

To optimize the extraction and purification procedure for polysaccharides from Astragalus membranaceus. The orthogonal test was used to determine the optimal extraction technology in terms of the extraction rate of crude polysaccharides and the concentration of polysaccharides. The efficiency of deproteinization and decoloration with different methods were evaluated with the ration of protein and colorant removing as the indices. Their influence on the content of Astragalus polysaccharides was calculated as well. The optimum extraction procedure was as follows: 8 times of water extracting three times at 100 degrees C, 90 minutes per time. Deproteinization using pepsase combined with Sevage solvent and depigmenting with DEAE fibrin achieved a high purification rate. The optimized extraction and purification procedure is simple, quick and efficient. The result establishes the foundation for the further study of the polysaccharides from Astragalus membranaceus.