针刺与埋线干预对抑郁大鼠中枢单胺类神经递质的影响

目的:探讨针刺与埋线治疗抑郁症的疗效差异和作用机制。方法:将32只成年雌雄各半的SD大鼠随机分为正常组、模型组、针刺组和埋线组,每组8只。后3组采用孤养和长期不可预见的中等强度刺激应激造成抑郁大鼠模型,同时针刺组和埋线组开始治疗,周期21天。采用高效液相色谱系统——电化学检测器检测大鼠脑内去甲肾上腺素(NE)、5-羟色胺(5-HT)和多巴胺(DA)的含量。结果:与正常组相比,模型大鼠下丘脑和海马的NE、5-HT和DA均有明显下降(P<0.05,P<0.01)。针刺与埋线均可使脑内的神经递质有不同程度的提高,埋线组在提高下丘脑的5-HT和DA以及海马的DA方面更具优势,但两组间比较差异无显著意义。结论:针刺与埋线对抑郁模型大鼠均有效,其作用机制具有相似性,均可通过调节中枢单胺类神经递质而发挥治疗作用。

OBJECTIVE: To compare therapeutic effects of acupuncture and embedding thread on depression and to probe the mechanism. METHODS: Thirty-two adult SD rats, 16 females and 16 males, were randomly divided into a normal group, a model group, an acupuncture group and an embedding thread group, 8 rats in each group. Separated feeding, long-term unpredictable and middle stimulation stress were used for development of depression rat model. At the same time, the treatment groups were treated for 21 days. The changes of norepinephrine (NE), 5-hydoxytryptamine (5-HT) and dopamine (DA) contents in the brain were detected by high performance liquid chromatography-electrochemical detector. RESULTS: Compared with the normal group, 5-HT, NE and DA contents in the hypothalamus and hippocampus decreased significantly in the model group (P < 0.05, P < 0.01); compared with the model group, the contents of the central monoamine neurotransmitters increased in both the acupuncture group and the embedding thread group, but the embedding thread group had more obvious action in improvement of 5-HT and DA levels in the hypothalamus and DA level in the hippocampus than the acupuncture group with no significant difference between the two groups. CONCLUSION: Both acupuncture and embedding thread therapy are effective for the depression model rat. They play the therapeutic role through regulating central monoamine neurotransmitters.