近红外染料 yb(p4) (cop) 掺杂PS/SiO 2复合纳米荧光标记物的制备及其性能

利用苯乙烯与3-甲基丙烯酰氧基丙基三甲氧基硅烷（KH570）化学反应合成共聚前驱物,再采用凝胶-溶胶法,与四乙氧基硅烷在一定的条件下共同水解与缩合,合成了近红外染料[（环戊二烯）钴（Ⅰ）三（二乙基亚磷酸基）][5,10,15,20-四（p-甲氧基苯基）-卟啉]合镱（Ⅲ）掺杂的聚苯乙烯/二氧化硅复合纳米粒子。这种制备染料掺杂复合纳米粒子的方法既克服了传统物理包埋方法染料容易泄漏的问题,又不受到化学键合方法对近红外染料分子性质的限制。用扫描电子显微镜表征该复合纳米粒子呈球形,大小均匀,直径约100nm。所制得的纳米粒子荧光性质稳定,受外界环境的影响小,具潜在生物亲和性,是一种新型的近红外荧光标记物。

In an effort to prepare efficient and uniform biolabels, we have developed a novel fluorophore doped polystyrene/silica nanoparticles via sol-gel method. The copolymer precursors are obtained via copolymerizations of styrene and 3-methactyloxylpropyltrimethoxylsilane (KH570). The controlled hydrolysis and condensation of tetraethyl orthosilicate (TEOS) in sol-gel route leads to the formation of near-infrared ytterbium(Ⅲ)- (cyclopentadienyl)tris(diethylphosphito)cobaltate(Ⅰ) anion dyes doped polystyrene/silica nanoparticles. The method significantly reduces fluorescent dye leaching from the nanoparticles, and can be conveniently applied to various dyes molecules with no active group. Scanning electron microscope(SEM) images showed that the nanoparticles are very uniform spherical shape and monodispersed with a diameter of about 100 nm. The present near-infrared fluorescent nanoparticles possess with the values of facilitating bio-modification, biocompatibility, higher detection sensitivity and photo-stabi-lity, and will be well applied in cell recognition, bacterial detection, DNA analysis and so on.