茶多酚锰合成、表征及络合和诱导肿瘤细胞凋亡的研究

合成了茶多酚锰(TP-Mn)和茶多酚锗(TP-Ge)，并采用红外光谱法及液相色谱质谱联用技术表征TP-Mn的理化特性。选用反向液相色谱(RP-HPLC)和电感藕合等离子体质谱(ICP-MS)技术研究牛血清白蛋白(BSA)络合TP-Mn的能力，指出BSA可直接络合于TP-Mn。采用MTT法研究TP和TP-Mn诱导Hela卵巢癌细胞的凋亡率。结果表明，TP和TP-Mn均能诱导Hela卵巢癌细胞凋亡，但TP-Mn诱导凋亡率约为TP的2倍。比较TP-Ge和TP-Mn诱导Raji人B淋巴瘤细胞凋亡率发现，两者诱导肿瘤细胞的凋亡率几乎相同，均高达86%左右。人血清白蛋白(HSB)可作为输送TP-Ge和TP-Mn的药物载体。

Both tea polyphenol-manganese(TP-Mn) and germanium(TP-Ge) were synthesized with organic synthesis approaches. The characterizations of TP-Mn were clearly revealed via infrared spectrometry and HPLC-ESI-Q-TOF mass spectrometry, respectively. Both HPLC and ICP-MS methods were employed to study the capacities for bovine serum albumen(BSA) binding to TP-Mn, indicating these capacities feasibility. Apoptosis rates in Hela ovarian induced with TP and TP-Mn were studied by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenly tetrazolium bromide(MTT) method. The experimental results show that both TP and TP-Mn can induced the cancer cell apoptosis, but the apoptosis rate with TP-Mn showed two times higher than that with TP. As compared experiments, the same apoptosis rates, going with up to approximately 86%, in Raji human B lymphoma induced with both TP and TP-Mn were observed directly. Human serum albumen might be employed to be a pharmacy carrier for transferring TP-Mn and TP-Ge in vivo.