新生鼠下丘脑结节乳头核组胺能神经细胞的原代培养

目的：探讨新生鼠下丘脑结节乳头核（TM）组胺能神经细胞的体外培养方法。方法：从新生鼠（24 h内）下丘脑后部分离出TM神经细胞，采用含有B27的Neurobasal Medium培养液进行体外原代培养。应用免疫细胞化学染色对培养的细胞进行鉴定。结果：从新生鼠下丘脑中分离的TM神经细胞，在本实验条件下生长良好。原代培养7〜9 d的神经细胞在形态上趋向成熟。免疫细胞化学染色结果表明培养的神经细胞呈组胺免疫反应阳性，为组胺能神经细胞。结论：新生鼠下丘脑TM神经细胞可进行体外原代培养，它可作为研究中枢组胺神经系统的体外细胞模型。

Objective: To establish primary cell culture conditions for tuberomammillary nucleus (TM) histaminergic neurons of neonatal rats. Methods: The tuberomammillary nucleus was dissected from the posterior part of the hypothalamus of neonatal rats, and Neurobasal medium supplemented with B27 was used for the primary cell culture. The cultured neurons were identified by immunocytochemical method (ICC). Results: TM neurons from the hypothalamus of neonatal rats were successfully cultured under the experimental condition and tended to mature morphologically on the days 7 to 9 of culture. The cultured neurons were stained by ICC and the green immunoreaction product was seen in the neurons under the fluorescence microscope, indicating that the cultured neurons were histamine-positive. Conclusion: TM neurons in the hypothalamus of neonatal rats can be cultured in vitro, and the primary cultured ones may serve as a cell model in vitro for the researches of central histaminergic system.