Abstract
Lactate dehydrogenase activities can be determined by using a fiber-optic biosensor based on visible absorption. In this method two dehydrogenate procedures were catalyzd by lactate dehydrogenase and diaphorase respectively, and coenzyme I (NAD+/NADH) was introduced into the two enzyme reactions as an electron transfer. The first dehydrogenate procedure is for the recognition of lactate dehydrogense and the second one will show change of absorbance. The sensing element of the biosonsor consists of draphorase covalence bound on the tip of the fiber bundle. A linear response of the biosensor was obtained in the range of 0-400 U/L of lactate dehydrogenase with a detection limit of 48 U/L. This biosensor has been used to determine lactate dehydrogenase activities in human serum.
| Translated title of the contribution | A fiber-optic biosensor based on visible absorption for the determination of lactate dehydrogenase activity |
|---|---|
| Original language | Chinese (Simplified) |
| Pages (from-to) | x10-1045 |
| Journal | 高等学校化学学报 |
| Volume | 18 |
| Issue number | 7 |
| Publication status | Published - 1997 |
Scopus Subject Areas
- Chemistry(all)
User-Defined Keywords
- Coenzyme I
- Dehydrogenase activity
- Diaphorase
- Fiber-optic biosensor
- Lactate dehydrogenase