基于可见吸收信号的乳酸脱氢酶光纤传感器

Translated title of the contribution: A fiber-optic biosensor based on visible absorption for the determination of lactate dehydrogenase activity

王柯敏*, 林朝晖, 蔡巍, 陈永康, 李慧明

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

Abstract

报道了一种测定乳酸脱氢酶活力的基于可见吸收信号的光纤生物传感器。在该传感体系中,通过辅酶I的氧化还原对(NAD+/NADH)将乳酸脱氢酶和心肌黄酶催化的两个脱氧过程予以耦合。第一个脱氢过程对分析对象进行化学识别,第二个脱氢过程引起可见吸收信号的变化。该传感器对0~400U/L的乳酸脱氢酶有线性响应关系,检测下限为48U/L。该传感器已用于人体血清中乳酸脱氢酶活力的测定。

Lactate dehydrogenase activities can be determined by using a fiber-optic biosensor based on visible absorption. In this method two dehydrogenate procedures were catalyzd by lactate dehydrogenase and diaphorase respectively, and coenzyme I (NAD+/NADH) was introduced into the two enzyme reactions as an electron transfer. The first dehydrogenate procedure is for the recognition of lactate dehydrogense and the second one will show  change of absorbance. The sensing element of the biosonsor consists of draphorase covalence bound on the tip of the fiber bundle. A linear response of the biosensor was obtained in the range of 0-400 U/L of lactate dehydrogenase with a detection limit of 48 U/L. This biosensor has been used to determine lactate dehydrogenase activities in human serum.

Translated title of the contributionA fiber-optic biosensor based on visible absorption for the determination of lactate dehydrogenase activity
Original languageChinese (Simplified)
Pages (from-to)1041-1045
Number of pages5
Journal高等学校化学学报
Volume18
Issue number7
Publication statusPublished - 24 Jul 1997

Scopus Subject Areas

  • Chemistry(all)

User-Defined Keywords

  • 光纤生物传感器
  • 脱氢酶活力
  • 乳酸脱氢酶
  • 心肌黄酶
  • 辅酶I
  • Fiber-optic biosensor
  • Dehydrogenase activity
  • Lactate dehydrogenase
  • Diaphorase
  • Coenzyme I

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