五指毛桃拮抗毒品可卡因的肝毒性作用及其活性成分研究

目的: 研究中药五指毛桃拮抗毒品可卡因的肝脏毒性作用及其活性成分。方法: 采用雄性ICR小鼠可卡因中毒模型。将ICR小鼠分别灌胃给予五指毛桃水煎剂,剂量分别相当于生药100,200,300 g.kg-1, 皮下注射盐酸可卡因染毒造模,测定血清中丙氨酸转移酶(ALT)、天冬氨酸转移酶(AST)和乳酸脱氢酶(LDH)的活性, 留取动物肝脏组织, 观察肝组织病理变化并测定肝组织过氧化氢酶(CAT)活性。通过活性追踪法寻找五指毛桃的活性成分, 并用相同方法对得到的化学成分抗可卡因肝毒性作用进行验证。结果:五指毛桃水煎剂能够使可卡因染毒小鼠血清转氨酶及过氧化氢酶含量呈剂量相关性降低, 且使肝组织有明显病理改变。补骨脂素作为五指毛桃的主要成分, 具有拮抗可卡因肝毒性的作用。结论: 五指毛桃可明显保护可卡因造成的肝损伤, 补骨脂素是其主要活性成分。

Objective: To investigate the protective effect of the roots of F.hirta against the cocaine-induced hepatotoxicity and it's active components. Method: Cocaine hydrochloride was subcutaneously injected to make male ICR mice liver wounded.Male ICR mice were randomly ig administered with the F.hirta decoction. The dose groups are 100,200,300 g·kg-1 herb materials per body weight.Cocaine hydrochloride was subcutaneously injected into the mice after the administration. The serum ALT, AST activity and the activity of CAT in liver homogenate were assayed, and liver change of pathomorphism was evaluated to prove the effect of the F.hirta decoction on cocaine-induced hepatotoxicity. And the activity of psoralean which was separated from the F.hirta decoction by bioassay-guided fractionation, was proofed in the same method. Result: We find that the F.hirta decoction shows a distinct effect on reducing serum transferase. The serum transferase and the content CAT in liver homogenate were dose-related reduced, and the histopathological examination found a significantly change of the liver tissues.And the psoralean, qua the mainly component, shows the same effect. Conclusion: F.hirta has the protective effect against the cocaine-induced hepatotoxicity. Psoralean is the basis.