中药龙血竭原植物剑叶龙血树的形态组织学研究及所含树脂的分布和成分检测

目的: 对剑叶龙血树进行系统的组织学研究及所含树脂的分布和成分检测研究, 为进一步研究剑叶龙血树树脂形成的成因和机理提供参考依据。方法: 采用彩色显微照相技术对剑叶龙血树含树脂茎和无树脂茎, 以及根、茎皮、叶进行系统的组织学研究; 同时用高效液相色谱仪将含树脂茎和无树脂茎的指纹图谱进行了比较。结果: 阐明了含树脂茎的纵切结构以及叶的表面观特征; 除了茎木质部导管和纤维中有树脂外, 发现剑叶龙血树受到损伤之后在茎皮层薄壁细胞、根的髓部和木质部亦有树脂的分布; 含树脂茎和无树脂茎的指纹图谱显示剑叶龙血树茎在形成树脂后的化学成分中出现了较多的黄酮类、茋类成分。结论:剑叶龙血树无分泌组织出现, 树脂出现在非分泌细胞中, 其树脂形成的机制需要进一步研究。

Objective: To study the anatomy of Dracaena cochinchinensis systematically, and find out the distribution and detect the constituents of its resin, in order to provide substantial foundation for the formation mechanism of its red resin.Method: The microscopic structures of D.cochinchinensis were systematically observed by using color micrographics, including stem with and without resin, roots, barks and leaves. The HPLC fingerprints of the stem with and without resin were compared. Result: Characteristics of the tangentical longitudinal section of stem with resin and surface view of leaves were elucidated.Besides xylem vessels and fibers of the stem, it was found that the red resin also exists in the cortex parenchyma cells of the stem and the medulla and xylem of the root. According to the HPLC fingerprint analysis result of the stems with and without resin, a number of flavones and stilbenoids were detected in the stem in which resin appeared after it wounded. Conclusion: No secretory tissue to secrete resin was found in D.cochinchinensis, further study is needed to elucidate the formation mechanism of its resin.