不同种源高良姜遗传多样性的AFLP分析

Translated title of the contribution: AFLP analysis of genetic diversity of Alpinia officinarum

杨全*, 张春荣, 陈虎彪, 滕希峰, 唐晓敏

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

目的: 研究不同种源高良姜的遗传多样性,探求各种质间的亲缘关系, 为合理利用高良姜种质资源及优良品种选育奠定理论基础。方法: 采用扩增片段长度多态性(AFLP)技术, 对8个种源地的高良姜进行遗传多态性分析, 将扩增出的条带作为原始矩阵, 用NTSYSpc-2.11F软件计算并分析高良姜种质间的相似度, 构建遗传系统进化树。结果:通过筛选得到8对AFLP引物组合, 共扩增出1 120个DNA条带, 多态性条带为1 044个, 多态性位点平均为92.57%; 通过构建系统进化树, 将8个种源地的高良姜种质分为3类。结论: 高良姜种质间存在较高多态性,遗传多样性丰富。

Objective: To explore the genetic diversity and relationship of different Alpinia officinarum germplasm.Method: Amplified fragment length polymorphism(AFLP) markers were developed to analyze genetic polymorphism in A.officinarum from eight resources.The amplified fragments were used as primary matrix with NTSYSpc-2.11F software to analyze the similarity between the A.officinarum germplasm and to construct the genetic phylogenetic tree.Result: A total of 1 120 fragments were genotyped using AFLP with eight prime combinations.Analysis identified 1 044 polymorphic fragments,accounting for 92.57% of the total detected variation.Genetic phylogenetic tree analysis indicates that three categories can be divided among the eight resources of A.officinarum.Conclusion: Significant polymorphism and genetic diversity can be observed among A.officinarum germplasm resources.

Translated title of the contributionAFLP analysis of genetic diversity of Alpinia officinarum
Original languageChinese (Simplified)
Pages (from-to)330-333
Number of pages4
Journal中国中药杂志
Volume36
Issue number3
DOIs
Publication statusPublished - 1 Feb 2011

Scopus Subject Areas

  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Complementary and alternative medicine
  • Pharmacology (medical)

User-Defined Keywords

  • 高良姜
  • 种质资源
  • 遗传多样性
  • Alpinia officinarum
  • Amplified fragment length polymorphism (AFLP)
  • Genetic diversity
  • Germplasm resources

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