Abstract
目的: 研究不同种源高良姜的遗传多样性,探求各种质间的亲缘关系, 为合理利用高良姜种质资源及优良品种选育奠定理论基础。方法: 采用扩增片段长度多态性(AFLP)技术, 对8个种源地的高良姜进行遗传多态性分析, 将扩增出的条带作为原始矩阵, 用NTSYSpc-2.11F软件计算并分析高良姜种质间的相似度, 构建遗传系统进化树。结果:通过筛选得到8对AFLP引物组合, 共扩增出1 120个DNA条带, 多态性条带为1 044个, 多态性位点平均为92.57%; 通过构建系统进化树, 将8个种源地的高良姜种质分为3类。结论: 高良姜种质间存在较高多态性,遗传多样性丰富。
Objective: To explore the genetic diversity and relationship of different Alpinia officinarum germplasm.Method: Amplified fragment length polymorphism(AFLP) markers were developed to analyze genetic polymorphism in A.officinarum from eight resources.The amplified fragments were used as primary matrix with NTSYSpc-2.11F software to analyze the similarity between the A.officinarum germplasm and to construct the genetic phylogenetic tree.Result: A total of 1 120 fragments were genotyped using AFLP with eight prime combinations.Analysis identified 1 044 polymorphic fragments,accounting for 92.57% of the total detected variation.Genetic phylogenetic tree analysis indicates that three categories can be divided among the eight resources of A.officinarum.Conclusion: Significant polymorphism and genetic diversity can be observed among A.officinarum germplasm resources.
| Translated title of the contribution | AFLP analysis of genetic diversity of Alpinia officinarum |
|---|---|
| Original language | Chinese (Simplified) |
| Pages (from-to) | 330-333 |
| Number of pages | 4 |
| Journal | 中国中药杂志 |
| Volume | 36 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 1 Feb 2011 |
Scopus Subject Areas
- Pharmacology, Toxicology and Pharmaceutics(all)
- Complementary and alternative medicine
- Pharmacology (medical)
User-Defined Keywords
- 高良姜
- 种质资源
- 遗传多样性
- Alpinia officinarum
- Amplified fragment length polymorphism (AFLP)
- Genetic diversity
- Germplasm resources