NAD capping of RNA: mechanism and functions

In this study, we will use E. coli and Arabidopsis as the model organisms to understand the molecular mechanisms that control NAD capping. Molecular and genetic approaches will be employed to reveal the molecular mode of action of some NAD-RNAs, particularly in transcriptional control. We will also investigate a unique mechanism in Arabidopsis that couples NAD capping/decapping with m7G capping and its role in mediating biological processes. This project will be carried out through multi-disciplinary collaboration with investigators who have complementary knowledge and skills in molecular biology, RNA biology, analytical chemistry, structural biology, biochemistry, and cell biology.